Mechanics of oxidative stress and protein turnover in C2C12 myotubes

Ya Tzu Chang

Mechanics of oxidative stress and protein turnover in C2C12 myotubes

Ya Tzu Chang

School of Molecular Sciences

Research output: Thesis › Master's Thesis

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Abstract

This study investigated the effects of hydrogen peroxide on the mechanics of protein turnover in C₂C₁₂ myotubes. The myotubes were treated with hydrogen peroxide modulating enzymes, catalase and glucose oxidase. Changes were observed in protein levels and protein turnover and the eiF2a pathway was found to be involved in these changes. A 2 tag method for thiol oxidation measurement was adapted to label the C₂C₁₂ cultures and was observed for the first time. Further studies could focus on the other possible protein target sites affected by oxidative stress in order to better understand muscle wasting Induced by oxidative stress.

Original language	English
Qualification	Masters
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Shavlakadze, Tea, Supervisor Arthur, Peter, Supervisor Grounds, Miranda, Supervisor
Award date	9 Dec 2016
Publication status	Unpublished - 2016

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THESIS - MASTER BY RESEARCH - CHANG Ya Tzu - 2016
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title = "Mechanics of oxidative stress and protein turnover in C2C12 myotubes",

abstract = "This study investigated the effects of hydrogen peroxide on the mechanics of protein turnover in C2C12 myotubes. The myotubes were treated with hydrogen peroxide modulating enzymes, catalase and glucose oxidase. Changes were observed in protein levels and protein turnover and the eiF2a pathway was found to be involved in these changes. A 2 tag method for thiol oxidation measurement was adapted to label the C2C12 cultures and was observed for the first time. Further studies could focus on the other possible protein target sites affected by oxidative stress in order to better understand muscle wasting Induced by oxidative stress.",

keywords = "Muscle wasting, Oxidative stress, Protein turnover, elF2a pathway, Thiol oxidation, Catalase, Glucose oxidase, Hydrogen peroxide",

author = "Chang, {Ya Tzu}",

year = "2016",

language = "English",

school = "The University of Western Australia",

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TY - THES

T1 - Mechanics of oxidative stress and protein turnover in C2C12 myotubes

AU - Chang, Ya Tzu

PY - 2016

Y1 - 2016

N2 - This study investigated the effects of hydrogen peroxide on the mechanics of protein turnover in C2C12 myotubes. The myotubes were treated with hydrogen peroxide modulating enzymes, catalase and glucose oxidase. Changes were observed in protein levels and protein turnover and the eiF2a pathway was found to be involved in these changes. A 2 tag method for thiol oxidation measurement was adapted to label the C2C12 cultures and was observed for the first time. Further studies could focus on the other possible protein target sites affected by oxidative stress in order to better understand muscle wasting Induced by oxidative stress.

AB - This study investigated the effects of hydrogen peroxide on the mechanics of protein turnover in C2C12 myotubes. The myotubes were treated with hydrogen peroxide modulating enzymes, catalase and glucose oxidase. Changes were observed in protein levels and protein turnover and the eiF2a pathway was found to be involved in these changes. A 2 tag method for thiol oxidation measurement was adapted to label the C2C12 cultures and was observed for the first time. Further studies could focus on the other possible protein target sites affected by oxidative stress in order to better understand muscle wasting Induced by oxidative stress.

KW - Muscle wasting

KW - Oxidative stress

KW - Protein turnover

KW - elF2a pathway

KW - Thiol oxidation

KW - Catalase

KW - Glucose oxidase

KW - Hydrogen peroxide

M3 - Master's Thesis

ER -

Mechanics of oxidative stress and protein turnover in C2C12 myotubes

Abstract

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