Mechanical behaviour of alginate-gelatin hydrogels for 3D bioprinting

Michael Di Giuseppe, Nicholas Law, Braeden Webb, Ryley A. Macrae, Lawrence J. Liew, Timothy B. Sercombe, Rodney J. Dilley, Barry J. Doyle

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Hydrogels comprised of alginate and gelatin have demonstrated potential as biomaterials in three dimensional (3D) bioprinting applications. However, as with all hydrogel-based biomaterials used in extrusion-based bioprinting, many parameters influence their performance and there is limited data characterising the behaviour of alginate-gelatin (Alg-Gel) hydrogels. Here we investigated nine Alg-Gel blends by varying the individual constituent concentrations. We tested samples for printability and print accuracy, compressive behaviour and change over time, and viability of encapsulated mesenchymal stem cells in bioprinted constructs. Printability tests showed a decrease in strand width with increasing concentrations of Alg-Gel. However due to the increased viscosity associated with the higher Alg-Gel concentrations, the minimum width was found to be 0.32 mm before blends became too viscous to print. Similarly, printing accuracy was increased in higher concentrations, exceeding 90% in some blends. Mechanical properties were assessed through uniaxial compression testing and it was found that increasing concentrations of both Alg and Gel resulted in higher compressive modulus. We also deemed 15 min crosslinking in calcium chloride to be sufficient. From our data, we propose a blend of 7%Alg-8%Gel that yields high printability, mechanical strength and stiffness, and cell viability. However, we found the compressive behaviour of Alg-Gel to reduce rapidly over time and especially when incubated at 37 °C. Here we have reported relevant data on Alg-Gel hydrogels for bioprinting. We tested for biomaterial properties and show that these hydrogels have many desirable characteristics that are highly tunable. Though further work is needed before practical use in vivo can be achieved.

Original languageEnglish
Pages (from-to)150-157
Number of pages8
JournalJournal of The Mechanical Behavior of Biomedical Materials
Volume79
DOIs
Publication statusPublished - 1 Mar 2018

Fingerprint

Hydrogels
Alginate
Gelatin
Biocompatible Materials
Biomaterials
Gels
Compression testing
Calcium Chloride
Calcium chloride
Hydrogel
alginic acid
Stem cells
Crosslinking
Strength of materials
Extrusion
Printing
Cells
Stiffness
Viscosity
Mechanical properties

Cite this

Giuseppe, Michael Di ; Law, Nicholas ; Webb, Braeden ; A. Macrae, Ryley ; Liew, Lawrence J. ; Sercombe, Timothy B. ; Dilley, Rodney J. ; Doyle, Barry J. / Mechanical behaviour of alginate-gelatin hydrogels for 3D bioprinting. In: Journal of The Mechanical Behavior of Biomedical Materials. 2018 ; Vol. 79. pp. 150-157.
@article{c606dbd32bb840d58529350e54278440,
title = "Mechanical behaviour of alginate-gelatin hydrogels for 3D bioprinting",
abstract = "Hydrogels comprised of alginate and gelatin have demonstrated potential as biomaterials in three dimensional (3D) bioprinting applications. However, as with all hydrogel-based biomaterials used in extrusion-based bioprinting, many parameters influence their performance and there is limited data characterising the behaviour of alginate-gelatin (Alg-Gel) hydrogels. Here we investigated nine Alg-Gel blends by varying the individual constituent concentrations. We tested samples for printability and print accuracy, compressive behaviour and change over time, and viability of encapsulated mesenchymal stem cells in bioprinted constructs. Printability tests showed a decrease in strand width with increasing concentrations of Alg-Gel. However due to the increased viscosity associated with the higher Alg-Gel concentrations, the minimum width was found to be 0.32 mm before blends became too viscous to print. Similarly, printing accuracy was increased in higher concentrations, exceeding 90{\%} in some blends. Mechanical properties were assessed through uniaxial compression testing and it was found that increasing concentrations of both Alg and Gel resulted in higher compressive modulus. We also deemed 15 min crosslinking in calcium chloride to be sufficient. From our data, we propose a blend of 7{\%}Alg-8{\%}Gel that yields high printability, mechanical strength and stiffness, and cell viability. However, we found the compressive behaviour of Alg-Gel to reduce rapidly over time and especially when incubated at 37 °C. Here we have reported relevant data on Alg-Gel hydrogels for bioprinting. We tested for biomaterial properties and show that these hydrogels have many desirable characteristics that are highly tunable. Though further work is needed before practical use in vivo can be achieved.",
keywords = "Alginate, Bioprinting, Characterisation, Gelatin, Hydrogel, Stem cells",
author = "Giuseppe, {Michael Di} and Nicholas Law and Braeden Webb and {A. Macrae}, Ryley and Liew, {Lawrence J.} and Sercombe, {Timothy B.} and Dilley, {Rodney J.} and Doyle, {Barry J.}",
year = "2018",
month = "3",
day = "1",
doi = "10.1016/j.jmbbm.2017.12.018",
language = "English",
volume = "79",
pages = "150--157",
journal = "Journal of The Mechanical Behavior of Biomedical Materials",
issn = "1751-6161",
publisher = "Elsevier",

}

Mechanical behaviour of alginate-gelatin hydrogels for 3D bioprinting. / Giuseppe, Michael Di; Law, Nicholas; Webb, Braeden; A. Macrae, Ryley; Liew, Lawrence J.; Sercombe, Timothy B.; Dilley, Rodney J.; Doyle, Barry J.

In: Journal of The Mechanical Behavior of Biomedical Materials, Vol. 79, 01.03.2018, p. 150-157.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Mechanical behaviour of alginate-gelatin hydrogels for 3D bioprinting

AU - Giuseppe, Michael Di

AU - Law, Nicholas

AU - Webb, Braeden

AU - A. Macrae, Ryley

AU - Liew, Lawrence J.

AU - Sercombe, Timothy B.

AU - Dilley, Rodney J.

AU - Doyle, Barry J.

PY - 2018/3/1

Y1 - 2018/3/1

N2 - Hydrogels comprised of alginate and gelatin have demonstrated potential as biomaterials in three dimensional (3D) bioprinting applications. However, as with all hydrogel-based biomaterials used in extrusion-based bioprinting, many parameters influence their performance and there is limited data characterising the behaviour of alginate-gelatin (Alg-Gel) hydrogels. Here we investigated nine Alg-Gel blends by varying the individual constituent concentrations. We tested samples for printability and print accuracy, compressive behaviour and change over time, and viability of encapsulated mesenchymal stem cells in bioprinted constructs. Printability tests showed a decrease in strand width with increasing concentrations of Alg-Gel. However due to the increased viscosity associated with the higher Alg-Gel concentrations, the minimum width was found to be 0.32 mm before blends became too viscous to print. Similarly, printing accuracy was increased in higher concentrations, exceeding 90% in some blends. Mechanical properties were assessed through uniaxial compression testing and it was found that increasing concentrations of both Alg and Gel resulted in higher compressive modulus. We also deemed 15 min crosslinking in calcium chloride to be sufficient. From our data, we propose a blend of 7%Alg-8%Gel that yields high printability, mechanical strength and stiffness, and cell viability. However, we found the compressive behaviour of Alg-Gel to reduce rapidly over time and especially when incubated at 37 °C. Here we have reported relevant data on Alg-Gel hydrogels for bioprinting. We tested for biomaterial properties and show that these hydrogels have many desirable characteristics that are highly tunable. Though further work is needed before practical use in vivo can be achieved.

AB - Hydrogels comprised of alginate and gelatin have demonstrated potential as biomaterials in three dimensional (3D) bioprinting applications. However, as with all hydrogel-based biomaterials used in extrusion-based bioprinting, many parameters influence their performance and there is limited data characterising the behaviour of alginate-gelatin (Alg-Gel) hydrogels. Here we investigated nine Alg-Gel blends by varying the individual constituent concentrations. We tested samples for printability and print accuracy, compressive behaviour and change over time, and viability of encapsulated mesenchymal stem cells in bioprinted constructs. Printability tests showed a decrease in strand width with increasing concentrations of Alg-Gel. However due to the increased viscosity associated with the higher Alg-Gel concentrations, the minimum width was found to be 0.32 mm before blends became too viscous to print. Similarly, printing accuracy was increased in higher concentrations, exceeding 90% in some blends. Mechanical properties were assessed through uniaxial compression testing and it was found that increasing concentrations of both Alg and Gel resulted in higher compressive modulus. We also deemed 15 min crosslinking in calcium chloride to be sufficient. From our data, we propose a blend of 7%Alg-8%Gel that yields high printability, mechanical strength and stiffness, and cell viability. However, we found the compressive behaviour of Alg-Gel to reduce rapidly over time and especially when incubated at 37 °C. Here we have reported relevant data on Alg-Gel hydrogels for bioprinting. We tested for biomaterial properties and show that these hydrogels have many desirable characteristics that are highly tunable. Though further work is needed before practical use in vivo can be achieved.

KW - Alginate

KW - Bioprinting

KW - Characterisation

KW - Gelatin

KW - Hydrogel

KW - Stem cells

UR - http://www.scopus.com/inward/record.url?scp=85039872380&partnerID=8YFLogxK

U2 - 10.1016/j.jmbbm.2017.12.018

DO - 10.1016/j.jmbbm.2017.12.018

M3 - Article

VL - 79

SP - 150

EP - 157

JO - Journal of The Mechanical Behavior of Biomedical Materials

JF - Journal of The Mechanical Behavior of Biomedical Materials

SN - 1751-6161

ER -