Measuring mechanodynamics in an unsupported epithelial monolayer grown at an air-water interface

Corinne Gullekson, Matthew Walker, James L. Harden, Andrew E. Pelling

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    Actomyosin contraction and relaxation in a monolayer is a fundamental biophysical process in development and homeostasis. Current methods used to characterize the mechanodynamics of monolayers often involve cells grown on solid supports such as glass or gels. The results of these studies are fundamentally influenced by these supporting . Here we describe a new method for measuring the mechanodynamics of epithelial monolayers by culturing cells at an air-liquid interface. These model monolayers are grown in the absence of any supporting structures, removing cell-substrate effects. This method's potential was evaluated by observing and quantifying the generation and release of internal stresses upon actomyosin contraction (800 ± 100 Pa) and relaxation (600 ± 100 Pa) in response to chemical treatments. Although unsupported monolayers exhibited clear major and minor strain axes, they were not correlated with nuclear alignment as observed when the monolayers were grown on soft deformable gels. It was also observed that both gels and glass substrates led to the promotion of long-range cell nuclei alignment not seen in the hanging-drop model. This new approach provides us with a picture of basal actomyosin mechanodynamics in a simplified system, allowing us to infer how the presence of a substrate affects contractility and long-range multicellular organization and dynamics.

    Original languageEnglish
    Pages (from-to)111-119
    Number of pages9
    JournalMolecular Biology of the Cell
    Volume28
    Issue number1
    DOIs
    Publication statusPublished - 1 Jan 2017

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    Actomyosin
    Gels
    Air
    Glass
    Biophysical Phenomena
    Water
    Cell Nucleus
    Homeostasis

    Cite this

    Gullekson, Corinne ; Walker, Matthew ; Harden, James L. ; Pelling, Andrew E. / Measuring mechanodynamics in an unsupported epithelial monolayer grown at an air-water interface. In: Molecular Biology of the Cell. 2017 ; Vol. 28, No. 1. pp. 111-119.
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    Measuring mechanodynamics in an unsupported epithelial monolayer grown at an air-water interface. / Gullekson, Corinne; Walker, Matthew; Harden, James L.; Pelling, Andrew E.

    In: Molecular Biology of the Cell, Vol. 28, No. 1, 01.01.2017, p. 111-119.

    Research output: Contribution to journalArticle

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