Measurement of nicotine and cotinine in human milk by high-performance liquid chromatography with ultraviolet absorbance detection

M. Page-Sharp, T.W. Hale, L.P. Hackett, J.H. Kristensen, Kenneth Ilett

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63 Citations (Scopus)

Abstract

A high-performance liquid chromatographic (HPLC) assay for the determination of nicotine and cotinine in human milk was developed using an extraction by liquid–liquid partition combined with back extraction into acid, and followed by reverse-phase chromatography with UV detection of analytes. The assay was linear up to 500 μg/l for both nicotine and cotinine. Intra- and inter-day relative standard deviations (R.S.D.) were
Original languageEnglish
Pages (from-to)173-180
JournalJournal of Chromatography B
Volume796
Issue number1
DOIs
Publication statusPublished - 2003

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