Measurement of nicotine and cotinine in human milk by high-performance liquid chromatography with ultraviolet absorbance detection

M. Page-Sharp; T.W. Hale; L.P. Hackett; J.H. Kristensen; Kenneth Ilett

doi:10.1016/j.jchromb.2003.08.020

Measurement of nicotine and cotinine in human milk by high-performance liquid chromatography with ultraviolet absorbance detection

M. Page-Sharp, T.W. Hale, L.P. Hackett, J.H. Kristensen, Kenneth Ilett

Pharmacology and Toxicology

Research output: Contribution to journal › Article › peer-review

64 Citations (Scopus)

Abstract

A high-performance liquid chromatographic (HPLC) assay for the determination of nicotine and cotinine in human milk was developed using an extraction by liquid–liquid partition combined with back extraction into acid, and followed by reverse-phase chromatography with UV detection of analytes. The assay was linear up to 500 μg/l for both nicotine and cotinine. Intra- and inter-day relative standard deviations (R.S.D.) were

Original language	English
Pages (from-to)	173-180
Journal	Journal of Chromatography B
Volume	796
Issue number	1
DOIs	https://doi.org/10.1016/j.jchromb.2003.08.020
Publication status	Published - 2003

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10.1016/j.jchromb.2003.08.020

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abstract = "A high-performance liquid chromatographic (HPLC) assay for the determination of nicotine and cotinine in human milk was developed using an extraction by liquid–liquid partition combined with back extraction into acid, and followed by reverse-phase chromatography with UV detection of analytes. The assay was linear up to 500 μg/l for both nicotine and cotinine. Intra- and inter-day relative standard deviations (R.S.D.) were",

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AU - Hale, T.W.

AU - Hackett, L.P.

AU - Kristensen, J.H.

AU - Ilett, Kenneth

PY - 2003

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JO - Journal of Chromatography B

JF - Journal of Chromatography B

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Measurement of nicotine and cotinine in human milk by high-performance liquid chromatography with ultraviolet absorbance detection

Abstract

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