Marker-assisted breeding for TaALMT1, a major gene conferring aluminium tolerance to wheat

B.J. Soto-Cerda, C. Inostroza-Blancheteau, M. Mathías, E. Peñaloza, J. Zuñiga, G. Muñoz, Zed Rengel, H. Salvo-Garrido

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Abstract

© 2015, Springer Science+Business Media Dordrecht. Aluminium toxicity in acid soils is the main limitation to crop production worldwide. In wheat (Triticum aestivum L.), the Al-activated malate transporter (TaALMT1) gene located on chromosome 4DL is associated with malate efflux and Al-tolerance. To introgress Al-tolerance from the breeding line CAR3911 into the high yielding Al-sensitive cultivar Kumpa-INIA, phenotypic and molecular characterizations of gene/QTL underlying Al-tolerance in CAR3911 followed by marker-assisted backcrossing (MAS-BC) were undertaken. Al-tolerant backcross (BC) lines were selected using the functional marker ALMT1-4 designed immediately upstream of the TaALMT1 coding region. Foreground and background selections using ALMT1-4 and microsatellite markers were conducted. Linkage and sequence analyses suggest that the TaALMT1 gene could underly the Al-tolerance in CAR3911, possessing the same promoter type (V) as the Al-tolerant genotypes Carazinho and ET8. The MAS-BC strategy allowed the selection of Al-tolerant lines with the smallest introgressed region (6 cM) on 4D and the highest recurrent parent genome (RPG) (98 %) covering 2 194 cM of the wheat genome. The homozygous BC3F2 line named Kumpa-INIA-TaALMT1 expressed a 3-fold higher Al-tolerance than its isogenic line Kumpa-INIA at 40 μM Al in the hydroponic solution, and similarly to CAR3911 and Carazinho. The MAS-BC strategy was successful for the introgression of the TaALMT1 gene into Kumpa-INIA in only three BC generations, shortening the breeding cycle to 24 months, which promises to increase wheat production and a greater yield stability in the acid soils of Southern Chile.
Original languageEnglish
Pages (from-to)83-91
JournalBiologia Plantarum
Volume59
Issue number1
DOIs
Publication statusPublished - 2015

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    Soto-Cerda, B. J., Inostroza-Blancheteau, C., Mathías, M., Peñaloza, E., Zuñiga, J., Muñoz, G., Rengel, Z., & Salvo-Garrido, H. (2015). Marker-assisted breeding for TaALMT1, a major gene conferring aluminium tolerance to wheat. Biologia Plantarum, 59(1), 83-91. https://doi.org/10.1007/s10535-014-0474-x