Marked variations in gut microbiota and some innate immune responses of fresh water crayfish, marron (Cherax cainii, Austin 2002) fed dietary supplementation of Clostridium butyricum

Md Javed Foysal, Thi Thu Thuy Nguyen, Md Reaz Chaklader, Muhammad A.B. Siddik, Chin Yen Tay, Ravi Fotedar, Sanjay Kumar Gupta

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This study aimed to investigate the effects of Clostridium butyricum as a dietary probiotic supplement in fishmeal based diet on growth, gut microbiota and immune performance of marron (Cherax cainii). Marron were randomly distributed into two different treatment groups, control and probiotic fed group. After 42 days of feeding trial, the results revealed a significant (P <0:05) increase in growth due to increase in number of moults in marron fed probiotics. The probiotic diet also significantly enhanced the total haemocyte counts (THC), lysozyme activity in the haemolymph and protein content of the tail muscle in marron. Compared to control, the 16S rRNA sequences data demonstrated an enrichment of bacterial diversity in the probiotic fed marron where significant increase of Clostridium abundance was observed. The abundance for crayfish pathogen Vibrio and Aeromonas were found to be significantly reduced post feeding with probiotic diet. Predicted metabolic pathway revealed an increased activity for the metabolism and absorption of carbohydrate, degradation of amino acid, fatty acid and toxic compounds, and biosynthesis of secondary metabolites. C. butyricum supplementation also significantly modulated the expression level of immune-responsive genes of marron post challenged with Vibrio mimicus. The overall results suggest that C. butyricum could be used as dietary probiotic supplement in marron aquaculture.

Original languageEnglish
Article number7553
JournalPEERJ
Volume2019
Issue number8
DOIs
Publication statusPublished - 2019

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Cherax
Clostridium butyricum
Clostridium
Astacoidea
Probiotics
Dietary Supplements
Fresh Water
crayfish
intestinal microorganisms
Innate Immunity
probiotics
dietary supplements
Water
Nutrition
Diet
Vibrio mimicus
diet
Aeromonas
Hemocytes
Aquaculture

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Foysal, Md Javed ; Nguyen, Thi Thu Thuy ; Chaklader, Md Reaz ; Siddik, Muhammad A.B. ; Tay, Chin Yen ; Fotedar, Ravi ; Gupta, Sanjay Kumar. / Marked variations in gut microbiota and some innate immune responses of fresh water crayfish, marron (Cherax cainii, Austin 2002) fed dietary supplementation of Clostridium butyricum. In: PEERJ. 2019 ; Vol. 2019, No. 8.
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abstract = "This study aimed to investigate the effects of Clostridium butyricum as a dietary probiotic supplement in fishmeal based diet on growth, gut microbiota and immune performance of marron (Cherax cainii). Marron were randomly distributed into two different treatment groups, control and probiotic fed group. After 42 days of feeding trial, the results revealed a significant (P <0:05) increase in growth due to increase in number of moults in marron fed probiotics. The probiotic diet also significantly enhanced the total haemocyte counts (THC), lysozyme activity in the haemolymph and protein content of the tail muscle in marron. Compared to control, the 16S rRNA sequences data demonstrated an enrichment of bacterial diversity in the probiotic fed marron where significant increase of Clostridium abundance was observed. The abundance for crayfish pathogen Vibrio and Aeromonas were found to be significantly reduced post feeding with probiotic diet. Predicted metabolic pathway revealed an increased activity for the metabolism and absorption of carbohydrate, degradation of amino acid, fatty acid and toxic compounds, and biosynthesis of secondary metabolites. C. butyricum supplementation also significantly modulated the expression level of immune-responsive genes of marron post challenged with Vibrio mimicus. The overall results suggest that C. butyricum could be used as dietary probiotic supplement in marron aquaculture.",
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Marked variations in gut microbiota and some innate immune responses of fresh water crayfish, marron (Cherax cainii, Austin 2002) fed dietary supplementation of Clostridium butyricum. / Foysal, Md Javed; Nguyen, Thi Thu Thuy; Chaklader, Md Reaz; Siddik, Muhammad A.B.; Tay, Chin Yen; Fotedar, Ravi; Gupta, Sanjay Kumar.

In: PEERJ, Vol. 2019, No. 8, 7553, 2019.

Research output: Contribution to journalArticle

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T1 - Marked variations in gut microbiota and some innate immune responses of fresh water crayfish, marron (Cherax cainii, Austin 2002) fed dietary supplementation of Clostridium butyricum

AU - Foysal, Md Javed

AU - Nguyen, Thi Thu Thuy

AU - Chaklader, Md Reaz

AU - Siddik, Muhammad A.B.

AU - Tay, Chin Yen

AU - Fotedar, Ravi

AU - Gupta, Sanjay Kumar

PY - 2019

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AB - This study aimed to investigate the effects of Clostridium butyricum as a dietary probiotic supplement in fishmeal based diet on growth, gut microbiota and immune performance of marron (Cherax cainii). Marron were randomly distributed into two different treatment groups, control and probiotic fed group. After 42 days of feeding trial, the results revealed a significant (P <0:05) increase in growth due to increase in number of moults in marron fed probiotics. The probiotic diet also significantly enhanced the total haemocyte counts (THC), lysozyme activity in the haemolymph and protein content of the tail muscle in marron. Compared to control, the 16S rRNA sequences data demonstrated an enrichment of bacterial diversity in the probiotic fed marron where significant increase of Clostridium abundance was observed. The abundance for crayfish pathogen Vibrio and Aeromonas were found to be significantly reduced post feeding with probiotic diet. Predicted metabolic pathway revealed an increased activity for the metabolism and absorption of carbohydrate, degradation of amino acid, fatty acid and toxic compounds, and biosynthesis of secondary metabolites. C. butyricum supplementation also significantly modulated the expression level of immune-responsive genes of marron post challenged with Vibrio mimicus. The overall results suggest that C. butyricum could be used as dietary probiotic supplement in marron aquaculture.

KW - Bioinformatics

KW - Gene expression profiling

KW - Health and immune indices

KW - High throughput sequencing

KW - Marron

KW - Probiotic bacteria

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