TY - JOUR
T1 - Mapping of Mitochondrial RNA-Protein Interactions by Digital RNase Footprinting
AU - Liu, G.
AU - Mercer, T.R.
AU - Shearwood, Anne-Marie
AU - Siira, Stefan
AU - Hibbs, Moira
AU - Mattick, J.S.
AU - Rackham, Oliver
AU - Filipovska, Aleksandra
PY - 2013/11/14
Y1 - 2013/11/14
N2 - Human mitochondrial DNA is transcribed as long polycistronic transcripts that encompass each strand of the genome and are processed subsequently into mature mRNAs, tRNAs, and rRNAs, necessitating widespread posttranscriptional regulation. Here, we establish methods for massively parallel sequencing and analyses of RNase-accessible regions of human mitochondrial RNA and thereby identify specific regions within mitochondrial transcripts that are bound by proteins. This approach provides a range of insights into the contribution of RNA-binding proteins to the regulation of mitochondrial gene expression.
AB - Human mitochondrial DNA is transcribed as long polycistronic transcripts that encompass each strand of the genome and are processed subsequently into mature mRNAs, tRNAs, and rRNAs, necessitating widespread posttranscriptional regulation. Here, we establish methods for massively parallel sequencing and analyses of RNase-accessible regions of human mitochondrial RNA and thereby identify specific regions within mitochondrial transcripts that are bound by proteins. This approach provides a range of insights into the contribution of RNA-binding proteins to the regulation of mitochondrial gene expression.
U2 - 10.1016/j.celrep.2013.09.036
DO - 10.1016/j.celrep.2013.09.036
M3 - Article
C2 - 24183674
VL - 5
SP - 839
EP - 848
JO - Cell Reports
JF - Cell Reports
SN - 2211-1247
IS - 3
ER -