Mapping of Mitochondrial RNA-Protein Interactions by Digital RNase Footprinting

G. Liu, T.R. Mercer, Anne-Marie Shearwood, Stefan Siira, Moira Hibbs, J.S. Mattick, Oliver Rackham, Aleksandra Filipovska

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)
268 Downloads (Pure)

Abstract

Human mitochondrial DNA is transcribed as long polycistronic transcripts that encompass each strand of the genome and are processed subsequently into mature mRNAs, tRNAs, and rRNAs, necessitating widespread posttranscriptional regulation. Here, we establish methods for massively parallel sequencing and analyses of RNase-accessible regions of human mitochondrial RNA and thereby identify specific regions within mitochondrial transcripts that are bound by proteins. This approach provides a range of insights into the contribution of RNA-binding proteins to the regulation of mitochondrial gene expression.
Original languageEnglish
Pages (from-to)839-848
JournalCell Reports
Volume5
Issue number3
DOIs
Publication statusPublished - 14 Nov 2013

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