Mapping-by-sequencing accelerates forward genetics in barley

Martin Mascher, Matthias Jost, Joel Elias Kuon, Axel Himmelbach, Axel Abfalg, Sebastian Beier, Uwe Scholz, Andreas Graner, Nils Stein

Research output: Contribution to journalArticlepeer-review

95 Citations (Scopus)


Mapping-by-sequencing has emerged as a powerful technique for genetic mapping in several plant and animal species. As this resequencing-based method requires a reference genome, its application to complex plant genomes with incomplete and fragmented sequence resources remains challenging. We perform exome sequencing of phenotypic bulks of a mapping population of barley segregating for a mutant phenotype that increases the rate of leaf initiation. Read depth analysis identifies a candidate gene, which is confirmed by the analysis of independent mutant alleles. Our method illustrates how the genomic resources of barley together with exome resequencing can underpin mapping-by-sequencing.

Original languageEnglish
Article numberR78
JournalGenome Biology
Issue number6
Publication statusPublished - 2014
Externally publishedYes


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