Manufactured Cell Blocks: Turning Smears into Sections

Alexandra Kang, Alina Miranda, Bastiaan De Boer

Research output: Contribution to journalArticle

Abstract

Whilst cytological smears are still the basis of cytodiagnosis, there is an increasing role for ancillary testing. Specimens obtained are not always optimal, often with limited material for ancillary studies. Several reports have described the utility of scraping material from cytological smears to manufacture cell blocks to provide material for ancillary studies. Our objective was a retrospective review of the PathWest (QE2) experience with manufactured cell blocks (mCB) over the last 10 years. A total of 178 fine-needle aspiration cases with mCB were extracted from the PathWest database. Data were subdivided into: lymph node (89), breast (31), thyroid (23), soft tissue (13), liver (11), and other sites (11) and were analysed. All available material was reviewed. Diagnostic material was identified in 163 mCB (91.6%). Immunohistochemistry (IHC) was performed on 149 cases. Positive IHC staining was seen in 139 cases (93.3%) and advanced the diagnosis in 119 cases (79.9%). Molecular studies were performed on 38 mCB with adequate DNA obtained in 37 cases (97.3%). Our review has demonstrated that cellular material scraped from air-dried or prefixed smears can be made into cell blocks. Antigen preservation is adequate to provide diagnostically useful results with IHC whilst DNA integrity is preserved to allow molecular analysis.

Original languageEnglish
Pages (from-to)28-34
Number of pages7
JournalACTA CYTOLOGICA
Volume63
Issue number1
DOIs
Publication statusPublished - 1 Feb 2019

Fingerprint

Immunohistochemistry
Cytodiagnosis
DNA
Fine Needle Biopsy
Thyroid Gland
Breast
Lymph Nodes
Air
Databases
Staining and Labeling
Antigens
Liver

Cite this

Kang, Alexandra ; Miranda, Alina ; De Boer, Bastiaan. / Manufactured Cell Blocks : Turning Smears into Sections. In: ACTA CYTOLOGICA. 2019 ; Vol. 63, No. 1. pp. 28-34.
@article{a39c538414e6428585015ff9733a335c,
title = "Manufactured Cell Blocks: Turning Smears into Sections",
abstract = "Whilst cytological smears are still the basis of cytodiagnosis, there is an increasing role for ancillary testing. Specimens obtained are not always optimal, often with limited material for ancillary studies. Several reports have described the utility of scraping material from cytological smears to manufacture cell blocks to provide material for ancillary studies. Our objective was a retrospective review of the PathWest (QE2) experience with manufactured cell blocks (mCB) over the last 10 years. A total of 178 fine-needle aspiration cases with mCB were extracted from the PathWest database. Data were subdivided into: lymph node (89), breast (31), thyroid (23), soft tissue (13), liver (11), and other sites (11) and were analysed. All available material was reviewed. Diagnostic material was identified in 163 mCB (91.6{\%}). Immunohistochemistry (IHC) was performed on 149 cases. Positive IHC staining was seen in 139 cases (93.3{\%}) and advanced the diagnosis in 119 cases (79.9{\%}). Molecular studies were performed on 38 mCB with adequate DNA obtained in 37 cases (97.3{\%}). Our review has demonstrated that cellular material scraped from air-dried or prefixed smears can be made into cell blocks. Antigen preservation is adequate to provide diagnostically useful results with IHC whilst DNA integrity is preserved to allow molecular analysis.",
keywords = "Cell block, Fine needle aspiration, Immunohistochemistry, Manufacture, Molecular studies, Scrape technique",
author = "Alexandra Kang and Alina Miranda and {De Boer}, Bastiaan",
year = "2019",
month = "2",
day = "1",
doi = "10.1159/000493907",
language = "English",
volume = "63",
pages = "28--34",
journal = "ACTA CYTOLOGICA",
issn = "0001-5547",
publisher = "Science Printers and Publishers",
number = "1",

}

Kang, A, Miranda, A & De Boer, B 2019, 'Manufactured Cell Blocks: Turning Smears into Sections' ACTA CYTOLOGICA, vol. 63, no. 1, pp. 28-34. https://doi.org/10.1159/000493907

Manufactured Cell Blocks : Turning Smears into Sections. / Kang, Alexandra; Miranda, Alina; De Boer, Bastiaan.

In: ACTA CYTOLOGICA, Vol. 63, No. 1, 01.02.2019, p. 28-34.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Manufactured Cell Blocks

T2 - Turning Smears into Sections

AU - Kang, Alexandra

AU - Miranda, Alina

AU - De Boer, Bastiaan

PY - 2019/2/1

Y1 - 2019/2/1

N2 - Whilst cytological smears are still the basis of cytodiagnosis, there is an increasing role for ancillary testing. Specimens obtained are not always optimal, often with limited material for ancillary studies. Several reports have described the utility of scraping material from cytological smears to manufacture cell blocks to provide material for ancillary studies. Our objective was a retrospective review of the PathWest (QE2) experience with manufactured cell blocks (mCB) over the last 10 years. A total of 178 fine-needle aspiration cases with mCB were extracted from the PathWest database. Data were subdivided into: lymph node (89), breast (31), thyroid (23), soft tissue (13), liver (11), and other sites (11) and were analysed. All available material was reviewed. Diagnostic material was identified in 163 mCB (91.6%). Immunohistochemistry (IHC) was performed on 149 cases. Positive IHC staining was seen in 139 cases (93.3%) and advanced the diagnosis in 119 cases (79.9%). Molecular studies were performed on 38 mCB with adequate DNA obtained in 37 cases (97.3%). Our review has demonstrated that cellular material scraped from air-dried or prefixed smears can be made into cell blocks. Antigen preservation is adequate to provide diagnostically useful results with IHC whilst DNA integrity is preserved to allow molecular analysis.

AB - Whilst cytological smears are still the basis of cytodiagnosis, there is an increasing role for ancillary testing. Specimens obtained are not always optimal, often with limited material for ancillary studies. Several reports have described the utility of scraping material from cytological smears to manufacture cell blocks to provide material for ancillary studies. Our objective was a retrospective review of the PathWest (QE2) experience with manufactured cell blocks (mCB) over the last 10 years. A total of 178 fine-needle aspiration cases with mCB were extracted from the PathWest database. Data were subdivided into: lymph node (89), breast (31), thyroid (23), soft tissue (13), liver (11), and other sites (11) and were analysed. All available material was reviewed. Diagnostic material was identified in 163 mCB (91.6%). Immunohistochemistry (IHC) was performed on 149 cases. Positive IHC staining was seen in 139 cases (93.3%) and advanced the diagnosis in 119 cases (79.9%). Molecular studies were performed on 38 mCB with adequate DNA obtained in 37 cases (97.3%). Our review has demonstrated that cellular material scraped from air-dried or prefixed smears can be made into cell blocks. Antigen preservation is adequate to provide diagnostically useful results with IHC whilst DNA integrity is preserved to allow molecular analysis.

KW - Cell block

KW - Fine needle aspiration

KW - Immunohistochemistry

KW - Manufacture

KW - Molecular studies

KW - Scrape technique

UR - http://www.scopus.com/inward/record.url?scp=85059979989&partnerID=8YFLogxK

U2 - 10.1159/000493907

DO - 10.1159/000493907

M3 - Article

VL - 63

SP - 28

EP - 34

JO - ACTA CYTOLOGICA

JF - ACTA CYTOLOGICA

SN - 0001-5547

IS - 1

ER -