Making a Pangenome Using the Iterative Mapping Approach

Cassandria Tay Fernandez

Research output: Chapter in Book/Conference paperChapterpeer-review

Abstract

Pangenomes have replaced single reference genomes as genetic references, as they contain a better scope of the diversity found in a single species. This protocol outlines the iterative mapping approach in constructing a pangenome, including how to check the raw data, align the data to a reference, how to assemble the data, and how to remove potential contaminants from the final assembly.

Original languageEnglish
Title of host publicationPlant Bioinformatics
Subtitle of host publicationMethods and Protocols
EditorsDavid Edwards
PublisherHumana Press
Chapter14
Pages259-271
Number of pages13
Volume2443
Edition3rd
ISBN (Electronic)978-1-0716-2067-0
ISBN (Print)978-1-0716-2066-3
DOIs
Publication statusPublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2443
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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