Lyn-Deficient Mice Develop Severe, Persistent Asthma: Lyn Is a Critical Negative Regulator of Th2 Immunity

S.J.E. Beavitt, K.W. Harder, J.M. Kemp, J. Jones, C. Quilici, F. Casagranda, E. Lam, Debra Turner, Siobhan Brennan, Peter Sly, D.M. Tarlinton, G.P. Anderson, M.L. Hibbs

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

The etiology of asthma, a chronic inflammatory disorder of the airways, remains obscure, although T cells appear to be central disease mediators. Lyn tyrosine kinase has been implicated as both a facilitator and inhibitor of signaling pathways that play a role in allergic inflammation, although its role in asthma is unclear because Lyn is not expressed in T cells. We show in the present study that Lyn(-/-) mice develop a severe, persistent inflammatory asthma-like syndrome with lung eosinophilia, mast cell hyperdegranulation, intensified bronchospasm, hyper IgE, and Th2-polarizing dendritic cells. Dendritic cells from Lyn(-/-) mice have a more immature phenotype, exhibit defective inhibitory signaling pathways, produce less IL-12, and can transfer disease when adoptively transferred into wild-type recipients. Our results show that Lyn regulates the intensity and duration of multiple asthmatic traits and indicate that Lyn is an important negative regulator of Th2 immune responses.
Original languageEnglish
Pages (from-to)1867-75
JournalJournal of Immunology
Volume175
Issue number3
DOIs
Publication statusPublished - 2005

    Fingerprint

Cite this

Beavitt, S. J. E., Harder, K. W., Kemp, J. M., Jones, J., Quilici, C., Casagranda, F., ... Hibbs, M. L. (2005). Lyn-Deficient Mice Develop Severe, Persistent Asthma: Lyn Is a Critical Negative Regulator of Th2 Immunity. Journal of Immunology, 175(3), 1867-75. https://doi.org/10.4049/jimmunol.175.3.1867