Loss of heterozygosity in endometrial carcinoma

Ana Velasco, Judit Pallares, Maria Santacana, Andre Yeramian, Xavier Dolcet, Nuria Eritja, Soraya Puente, Anabel Sorolla, Nuria Llecha, Xavier Matias-Guiu

Research output: Contribution to journalReview article

18 Citations (Scopus)

Abstract

Inactivation of a tumor suppressor gene typically occurs in two steps, thus fulfilling Knudson hypothesis. One "hit" is frequently a point mutation or a small deletion. The other alteration is usually a large genomic loss of part of a gene, or even part of a chromosome, or the whole chromosome. However, it is not clear which of these two events occurs first. Loss of heterozygosity (LOH) analysis allows the identification of one of the 2 hits. Although microsatellite polymerase chain reaction is the technique most frequently used to assess LOH, other different approaches can also be used. The LOH can also be assessed by restriction fragment length polymorphism analysis, single strand conformation polymorphism analysis, oligonucleotide microarrays capable to simultaneously determine the genotype of thousands of single-nucleotide polymorphism (single-nucleotide polymorphism arrays), comparative genomic hybridization, multiplex amplification and probe hybridization, and multiplex ligation-dependent probe amplification. In this article, the authors review the results obtained with molecular analysis of LOH in the understanding of development and progression of endometrial carcinoma. Particular attention is given to: (1) the presence of widespread LOH in nonendometrioid carcinoma, probably reflecting the existence of chromosomal instability; and (2) specific LOH patterns associated with some clinicopathologic features.

Original languageEnglish
Pages (from-to)305-317
Number of pages13
JournalInternational Journal of Gynecological Pathology
Volume27
Issue number3
DOIs
Publication statusPublished - Jul 2008

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