Listerial species escape from the phagosomes of interleukin-4-deactivated human macrophages independent of listeriolysin

K. Neumann, E. Eppler, Luis Filgueira, P. Groscurth, E. Gasal, A. Schaffner, G. Schoedon, M. Schneemann

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    Listeria monocytogenes is the causative agent of infections like sepsis and meningitis, especially in immunocompromised hosts. Human macrophages are able to phagocytose and digest L. monocytogenes but IL-4 prevents human macrophages from killing the bacteria, the mechanisms of which are unknown. In the present study, we examined various listeria species and strains including wild-type and deletion mutants in human macrophages pretreated with IL-4. To analyse the IL-4-mediated deactivation process, we combined quantitative infection assays with various morphologic methods. IL-4 facilitates survival and escape of the pathogenic L. monocytogenes wild-type strain 10403S from the macrophage phagosomes. In untreated macrophages, the isogenic listeriolysin deletion mutant strain DP-L2161 was killed and did not escape from the phagolysosomes. However, after macrophage deactivation with IL-4 DP-L2161 survived and escaped from the phagosomes. This was also the case, but to a lesser extent, even for the naturally avirulent L. innocua. As detected by confocal laser-scanning fluorescence microscopy and electron microscopy, IL-4 permitted the escape of all listeria species tested, including DP-L2161 and L. innocua from the phagosomal compartment of the macrophages. We conclude that escape from the phagosome and survival of the listeria species tested in IL-4-deactivated human macrophages is independent of the virulence factor listeriolysin.
    Original languageEnglish
    Pages (from-to)431-439
    JournalImmunology and Cell Biology
    Volume81
    DOIs
    Publication statusPublished - 2003

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    Phagosomes
    Interleukin-4
    Macrophages
    Listeria
    Listeria monocytogenes
    Survival
    Listeria monocytogenes hlyA protein
    Immunocompromised Host
    Virulence Factors
    Infection
    Meningitis
    Fluorescence Microscopy
    Phagocytosis
    Confocal Microscopy
    Sepsis
    Electron Microscopy
    Bacteria

    Cite this

    Neumann, K. ; Eppler, E. ; Filgueira, Luis ; Groscurth, P. ; Gasal, E. ; Schaffner, A. ; Schoedon, G. ; Schneemann, M. / Listerial species escape from the phagosomes of interleukin-4-deactivated human macrophages independent of listeriolysin. In: Immunology and Cell Biology. 2003 ; Vol. 81. pp. 431-439.
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    abstract = "Listeria monocytogenes is the causative agent of infections like sepsis and meningitis, especially in immunocompromised hosts. Human macrophages are able to phagocytose and digest L. monocytogenes but IL-4 prevents human macrophages from killing the bacteria, the mechanisms of which are unknown. In the present study, we examined various listeria species and strains including wild-type and deletion mutants in human macrophages pretreated with IL-4. To analyse the IL-4-mediated deactivation process, we combined quantitative infection assays with various morphologic methods. IL-4 facilitates survival and escape of the pathogenic L. monocytogenes wild-type strain 10403S from the macrophage phagosomes. In untreated macrophages, the isogenic listeriolysin deletion mutant strain DP-L2161 was killed and did not escape from the phagolysosomes. However, after macrophage deactivation with IL-4 DP-L2161 survived and escaped from the phagosomes. This was also the case, but to a lesser extent, even for the naturally avirulent L. innocua. As detected by confocal laser-scanning fluorescence microscopy and electron microscopy, IL-4 permitted the escape of all listeria species tested, including DP-L2161 and L. innocua from the phagosomal compartment of the macrophages. We conclude that escape from the phagosome and survival of the listeria species tested in IL-4-deactivated human macrophages is independent of the virulence factor listeriolysin.",
    author = "K. Neumann and E. Eppler and Luis Filgueira and P. Groscurth and E. Gasal and A. Schaffner and G. Schoedon and M. Schneemann",
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    Neumann, K, Eppler, E, Filgueira, L, Groscurth, P, Gasal, E, Schaffner, A, Schoedon, G & Schneemann, M 2003, 'Listerial species escape from the phagosomes of interleukin-4-deactivated human macrophages independent of listeriolysin' Immunology and Cell Biology, vol. 81, pp. 431-439. https://doi.org/10.1046/j.1440-1711.2003.01196.x

    Listerial species escape from the phagosomes of interleukin-4-deactivated human macrophages independent of listeriolysin. / Neumann, K.; Eppler, E.; Filgueira, Luis; Groscurth, P.; Gasal, E.; Schaffner, A.; Schoedon, G.; Schneemann, M.

    In: Immunology and Cell Biology, Vol. 81, 2003, p. 431-439.

    Research output: Contribution to journalArticle

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    T1 - Listerial species escape from the phagosomes of interleukin-4-deactivated human macrophages independent of listeriolysin

    AU - Neumann, K.

    AU - Eppler, E.

    AU - Filgueira, Luis

    AU - Groscurth, P.

    AU - Gasal, E.

    AU - Schaffner, A.

    AU - Schoedon, G.

    AU - Schneemann, M.

    PY - 2003

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    AB - Listeria monocytogenes is the causative agent of infections like sepsis and meningitis, especially in immunocompromised hosts. Human macrophages are able to phagocytose and digest L. monocytogenes but IL-4 prevents human macrophages from killing the bacteria, the mechanisms of which are unknown. In the present study, we examined various listeria species and strains including wild-type and deletion mutants in human macrophages pretreated with IL-4. To analyse the IL-4-mediated deactivation process, we combined quantitative infection assays with various morphologic methods. IL-4 facilitates survival and escape of the pathogenic L. monocytogenes wild-type strain 10403S from the macrophage phagosomes. In untreated macrophages, the isogenic listeriolysin deletion mutant strain DP-L2161 was killed and did not escape from the phagolysosomes. However, after macrophage deactivation with IL-4 DP-L2161 survived and escaped from the phagosomes. This was also the case, but to a lesser extent, even for the naturally avirulent L. innocua. As detected by confocal laser-scanning fluorescence microscopy and electron microscopy, IL-4 permitted the escape of all listeria species tested, including DP-L2161 and L. innocua from the phagosomal compartment of the macrophages. We conclude that escape from the phagosome and survival of the listeria species tested in IL-4-deactivated human macrophages is independent of the virulence factor listeriolysin.

    U2 - 10.1046/j.1440-1711.2003.01196.x

    DO - 10.1046/j.1440-1711.2003.01196.x

    M3 - Article

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    JO - Immunology & Cell Biology

    JF - Immunology & Cell Biology

    SN - 0818-9641

    ER -