Lipopolysaccharide Structure and Biosynthesis in Helicobacter pylori

Hong Li, Tingting Liao, Aleksandra Debowski, Hong Tang, Hans Olof Nilsson, Keith A. Stubbs, Barry J. Marshall, Mohammed Benghezal

Research output: Contribution to journalReview article

12 Citations (Scopus)
150 Downloads (Pure)

Abstract

This review covers the current knowledge and gaps in Helicobacter pylori lipopolysaccharide (LPS) structure and biosynthesis. H. pylori is a Gram-negative bacterium which colonizes the luminal surface of the human gastric epithelium. Both a constitutive alteration of the lipid A preventing TLR4 elicitation and host mimicry of the Lewis antigen decorated O-antigen of H. pylori LPS promote immune escape and chronic infection. To date, the complete structure of H. pylori LPS is not available, and the proposed model is a linear arrangement composed of the inner core defined as the hexa-saccharide (Kdo-LD-Hep-LD-Hep-DD-Hep-Gal-Glc), the outer core composed of a conserved trisaccharide (-GlcNAc-Fuc-DD-Hep-) linked to the third heptose of the inner core, the glucan, the heptan and a variable O-antigen, generally consisting of a poly-LacNAc decorated with Lewis antigens. Although the glycosyltransferases (GTs) responsible for the biosynthesis of the H. pylori O-antigen chains have been identified and characterized, there are many gaps in regard to the biosynthesis of the core LPS. These limitations warrant additional mutagenesis and structural studies to obtain the complete LPS structure and corresponding biosynthetic pathway of this important gastric bacterium.

Original languageEnglish
Pages (from-to)445-461
Number of pages17
JournalHelicobacter
Volume21
Issue number6
Early online date15 Nov 2016
DOIs
Publication statusPublished - 1 Dec 2016

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O Antigens
Helicobacter pylori
Lipopolysaccharides
Stomach
Heptoses
Molecular Mimicry
Trisaccharides
Glycosyltransferases
Lipid A
Glucans
Biosynthetic Pathways
Gram-Negative Bacteria
Mutagenesis
Epithelium
Bacteria
Antigens
Infection
Helicobacter pylori lipopolysaccharide

Cite this

Li, Hong ; Liao, Tingting ; Debowski, Aleksandra ; Tang, Hong ; Nilsson, Hans Olof ; Stubbs, Keith A. ; Marshall, Barry J. ; Benghezal, Mohammed. / Lipopolysaccharide Structure and Biosynthesis in Helicobacter pylori. In: Helicobacter. 2016 ; Vol. 21, No. 6. pp. 445-461.
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Lipopolysaccharide Structure and Biosynthesis in Helicobacter pylori. / Li, Hong; Liao, Tingting; Debowski, Aleksandra; Tang, Hong; Nilsson, Hans Olof; Stubbs, Keith A.; Marshall, Barry J.; Benghezal, Mohammed.

In: Helicobacter, Vol. 21, No. 6, 01.12.2016, p. 445-461.

Research output: Contribution to journalReview article

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