Lipidomics reveals associations of phospholipids with obesity and insulin resistance in young adults

S. Rauschert, O. Uhl, B. Koletzko, F. Kirchberg, Trevor Mori, Rae-Chi Huang, Lawrence Beilin, C. Hellmuth, Wendy Oddy

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

© 2016 by the Endocrine Society. Context: Obesity and related diseases have become a global public health burden. Identifying biomarkers will lead to a better understanding of the underlying mechanisms associated with obesity and the pathways leading to insulin resistance (IR) and diabetes. Objective: This study aimed to identify the lipidomic biomarkers associated with obesity and IR using plasma samples from a population-based cohort of young adults. Design and Setting: The Western Australian Pregnancy Cohort (Raine) study enrolled 2900 pregnant women from 1989 to 1991. The 20-year follow-up was conducted between March 2010 and April 2012. Participants and Samples: Plasma samples from 1176 subjects aged 20 years were analyzed using mass spectrometry-based metabolomics. Main Outcome Measures: Associations of analytes with markers of obesity and IR including body mass index, waist circumference, homeostasis model assessment (HOMA-IR), and insulin were examined. Analyses were stratified by body mass index and adjusted for lifestyle and other factors. Results: Waist circumference was positively associated with seven sphingomyelins and five diacylphosphatidylcholines and negatively associated with two lysophosphatidylcholines. HOMA-IR was negatively associated with two diacylphosphatidylcholines and positively with one lysophosphatidylcholine and one diacylphosphatidylcholine. No significant association was found in the obese/overweight group of the HOMA-IR model. In the normal-weight group, one lysophosphatidylcholine was increased. Conclusion: A possible discriminative effect of sphingomyelins, particularly those with two double bonds, and lysophosphatidylcholines was identified between subjects with normal weight and obesity independent of low-density lipoprotein cholesterol and high-density lipoprotein cholesterol concentrations. Our results suggest weight status-dependent mechanisms for the developmentof IR with lysophosphatidylcholine C14:0 as a key metabolite innonobeseIR.
Original languageEnglish
Pages (from-to)871-879
Number of pages9
JournalJournal of Clinical Endocrinology and Metabolism
Volume101
Issue number4
Early online date28 Dec 2015
DOIs
Publication statusPublished - 1 Mar 2016

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