TY - JOUR
T1 - Linking phytohormones with growth, transport activity and metabolic responses to cadmium in tomato
AU - Chu, Zhuannan
AU - Munir, Shoaib
AU - Zhao, Gangjun
AU - Hou, Jingdong
AU - Du, Wenqing
AU - Li, Ning
AU - Lu, Yongen
AU - Yu, Qinghui
AU - Shabala, Sergey
AU - Ouyang, Bo
N1 - Funding Information:
We thank Dr. Lázaro E. P. Peres for providing us hormone mutants seed. This work was supported by the National Key Research and Development Program of China (2018YFD1000800), and the National Natural Science Foundation of China (U1503186, 31572133).
Publisher Copyright:
© 2020, Springer Nature B.V.
PY - 2020/4/1
Y1 - 2020/4/1
N2 - This study investigated the role of phytohormones in the mediating effect of cadmium on growth and metabolism of tomato plants. Micro-Tom plants and its hormone mutants, procera (pro), diageotropica (dgt), epinastic (epi), and notabilis (not) have been used. Tomato seedlings were grown for 5 weeks under controlled hydroponics condition and then treated with CdCl2 concentration ranging from 0 to 100 μM for 2- to 6-week. Lipid peroxidation, leaf chlorophyll, elemental (Cd, Fe, Zn, Ca, Mg) content, and antioxidant enzyme activity were determined. Cd stress considerably altered the transcriptional level of the metal transport-related genes. Cadmium inhibited the uptake of iron (Fe) and zinc (Zn), whereas it has no noticeable effect on magnesium (Mg) and calcium (Ca) content, which might have been induced by the defense against Cd toxicity. The overall results demonstrated that not and epi mutants (with altered levels of ABA and ethylene, respectively) had a Cd-sensitive phenotype while dgt mutant (with reduced sensitivity to auxin) avoided damaging effects of Cd stress. This observation is explained by the lowest Cd accumulation in dgt roots. These results provide valuable information for the relative importance of auxin, ethylene, and abscisic acid in regulating plant responses to heavy metal toxicity.
AB - This study investigated the role of phytohormones in the mediating effect of cadmium on growth and metabolism of tomato plants. Micro-Tom plants and its hormone mutants, procera (pro), diageotropica (dgt), epinastic (epi), and notabilis (not) have been used. Tomato seedlings were grown for 5 weeks under controlled hydroponics condition and then treated with CdCl2 concentration ranging from 0 to 100 μM for 2- to 6-week. Lipid peroxidation, leaf chlorophyll, elemental (Cd, Fe, Zn, Ca, Mg) content, and antioxidant enzyme activity were determined. Cd stress considerably altered the transcriptional level of the metal transport-related genes. Cadmium inhibited the uptake of iron (Fe) and zinc (Zn), whereas it has no noticeable effect on magnesium (Mg) and calcium (Ca) content, which might have been induced by the defense against Cd toxicity. The overall results demonstrated that not and epi mutants (with altered levels of ABA and ethylene, respectively) had a Cd-sensitive phenotype while dgt mutant (with reduced sensitivity to auxin) avoided damaging effects of Cd stress. This observation is explained by the lowest Cd accumulation in dgt roots. These results provide valuable information for the relative importance of auxin, ethylene, and abscisic acid in regulating plant responses to heavy metal toxicity.
KW - Antioxidant enzyme
KW - Cadmium
KW - Chlorophyll
KW - Gene expression
UR - https://www.scopus.com/pages/publications/85078914471
U2 - 10.1007/s10725-020-00580-w
DO - 10.1007/s10725-020-00580-w
M3 - Article
AN - SCOPUS:85078914471
SN - 0167-6903
VL - 90
SP - 557
EP - 569
JO - Plant Growth Regulation
JF - Plant Growth Regulation
IS - 3
ER -