Linking oxygen availability with membrane potential maintenance and K+ retention of barley roots: Implications for waterlogging stress tolerance

F. Zeng, Dennis Konnerup, L. Shabala, M. Zhou, Tim Colmer, G. Zhang, S.N. Shabala

    Research output: Contribution to journalArticle

    24 Citations (Scopus)

    Abstract

    © 2014 John Wiley & Sons Ltd. Oxygen deprivation is a key determinant of root growth and functioning under waterlogging. In this work, changes in net K+ flux and membrane potential (MP) of root cells were measured from elongation and mature zones of two barley varieties under hypoxia and anoxia conditions in the medium, and as influenced by ability to transport O2 from the shoot. We show that O2 deprivation results in an immediate K+ loss from roots, in a tissue- and time-specific manner, affecting root K+ homeostasis. Both anoxia and hypoxia induced transient membrane depolarization; the extent of this depolarization varied depending on severity of O2 stress and was less pronounced in a waterlogging-tolerant variety. Intact roots of barley were capable of maintaining H+-pumping activity under hypoxic conditions while disrupting O2 transport from shoot to root resulted in more pronounced membrane depolarization under O2-limited conditions and in anoxia a rapid loss of the cell viability. It is concluded that the ability of root cells to maintain MP and cytosolic K+ homeostasis is central to plant performance under waterlogging, and efficient O2 transport from the shoot may enable operation of the plasma membrane H+-ATPase in roots even under conditions of severe O2 limitation in the soil solution.
    Original languageEnglish
    Pages (from-to)2325-2338
    Number of pages14
    JournalPlant, Cell and Environment
    Volume37
    Issue number10
    DOIs
    Publication statusPublished - 18 Aug 2014

    Fingerprint

    flooded conditions
    Hordeum
    membrane potential
    stress tolerance
    Membrane Potentials
    barley
    Maintenance
    Oxygen
    oxygen
    hypoxia
    Homeostasis
    shoots
    homeostasis
    Proton-Translocating ATPases
    Membranes
    H-transporting ATP synthase
    Cell Survival
    Soil
    Cell Membrane
    Hypoxia

    Cite this

    Zeng, F. ; Konnerup, Dennis ; Shabala, L. ; Zhou, M. ; Colmer, Tim ; Zhang, G. ; Shabala, S.N. / Linking oxygen availability with membrane potential maintenance and K+ retention of barley roots: Implications for waterlogging stress tolerance. In: Plant, Cell and Environment. 2014 ; Vol. 37, No. 10. pp. 2325-2338.
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    Linking oxygen availability with membrane potential maintenance and K+ retention of barley roots: Implications for waterlogging stress tolerance. / Zeng, F.; Konnerup, Dennis; Shabala, L.; Zhou, M.; Colmer, Tim; Zhang, G.; Shabala, S.N.

    In: Plant, Cell and Environment, Vol. 37, No. 10, 18.08.2014, p. 2325-2338.

    Research output: Contribution to journalArticle

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    AU - Konnerup, Dennis

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    AB - © 2014 John Wiley & Sons Ltd. Oxygen deprivation is a key determinant of root growth and functioning under waterlogging. In this work, changes in net K+ flux and membrane potential (MP) of root cells were measured from elongation and mature zones of two barley varieties under hypoxia and anoxia conditions in the medium, and as influenced by ability to transport O2 from the shoot. We show that O2 deprivation results in an immediate K+ loss from roots, in a tissue- and time-specific manner, affecting root K+ homeostasis. Both anoxia and hypoxia induced transient membrane depolarization; the extent of this depolarization varied depending on severity of O2 stress and was less pronounced in a waterlogging-tolerant variety. Intact roots of barley were capable of maintaining H+-pumping activity under hypoxic conditions while disrupting O2 transport from shoot to root resulted in more pronounced membrane depolarization under O2-limited conditions and in anoxia a rapid loss of the cell viability. It is concluded that the ability of root cells to maintain MP and cytosolic K+ homeostasis is central to plant performance under waterlogging, and efficient O2 transport from the shoot may enable operation of the plasma membrane H+-ATPase in roots even under conditions of severe O2 limitation in the soil solution.

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