TY - JOUR
T1 - Lightsheet fluorescence lifetime imaging microscopy (FLIM) with wide-field time-correlated single photon counting (TCSPC)
AU - Hirvonen, Liisa M
AU - Nedbal, Jakub
AU - Almutairi, Norah
AU - Phillips, Thomas A
AU - Becker, Wolfgang
AU - Conneely, Thomas
AU - Milnes, James
AU - Cox, Susan
AU - Stürzenbaum, Stephen
AU - Suhling, Klaus
PY - 2020/2/1
Y1 - 2020/2/1
N2 - We report onwide-field time-correlated single photon counting (TCSPC)-based fluorescence lifetime imagingmicroscopy (FLIM) with lightsheet illumination.A pulsed diode laser is used for excitation, and a crossed delay line anode image intensifier, effectively a single-photon sensitive camera, is used to record the position and arrival time of the photons with picosecond time resolution, combining low illumination intensity of microwattswith wide-field data collection.We pair this detector with the lightsheet illumination technique, and apply it to 3D FLIM imaging of dye gradients in human cancer cell spheroids, and C. elegans. This article is protected by copyright. All rights reserved.
AB - We report onwide-field time-correlated single photon counting (TCSPC)-based fluorescence lifetime imagingmicroscopy (FLIM) with lightsheet illumination.A pulsed diode laser is used for excitation, and a crossed delay line anode image intensifier, effectively a single-photon sensitive camera, is used to record the position and arrival time of the photons with picosecond time resolution, combining low illumination intensity of microwattswith wide-field data collection.We pair this detector with the lightsheet illumination technique, and apply it to 3D FLIM imaging of dye gradients in human cancer cell spheroids, and C. elegans. This article is protected by copyright. All rights reserved.
U2 - 10.1002/jbio.201960099
DO - 10.1002/jbio.201960099
M3 - Article
C2 - 31661595
SN - 1864-0648
VL - 13
JO - Journal of Biophotonics
JF - Journal of Biophotonics
IS - 2
M1 - e201960099
ER -
