Large-scale micropropagation of the Australian key species Gahnia radula (Cyperaceae) and its return to revegetation sites

A. Kodym, I. Clarke, C. Aponte, Shane Turner, Eric Bunn, J.C. Delpratt

    Research output: Contribution to journalArticle

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    Abstract

    © 2014 CSIRO. We report on the successful propagation of the sedge Gahnia radula (R.Br.) Benth. from seed by using plant tissue culture, and its successful establishment in the field. This keystone species, although common along parts of the eastern coast of Australia, is currently not available for revegetation because of a lack of efficient propagation methods, leading to the use of substitute species in many restoration programs. Even though seed quality is a common problem for G. radula, one population bearing filled seed was located in the near-east of Melbourne and after harvest of fruit in December 2011, seeds were successfully germinated in vitro after removal of the pericarp. Overnight soaking in sterile 10% (v/v) smoke water before culturing enhanced in vitro germination from 29.2% to 66.7%. In vitro-grown seedlings were then used as starting material for tissue-culture propagation via shoot culture. A micropropagation rate of about six new plantlets per cycle was achieved within 5-6 weeks with liquid half-strength Murashige-Skoog medium and a pulse treatment with 10 M 6-benzylaminopurine (BAP) and 2 M naphthalene acetic acid (NAA). Plants rooted after receiving a pulse treatment with 5 M kinetin and were successfully acclimatised into potting mix and were ready for field planting after 5-6 months. Tube stock was planted into two field sites with minimal weed control. Survival was 98% in both cases 1 month after planting and 54% and 74% after the summer. Division of in vitro-derived plants in the nursery was very successful, with 93-96% establishment of divisions. This research highlights the important role of plant tissue culture in conserving biodiversity of native flora.
    Original languageEnglish
    Pages (from-to)417-427
    JournalAustralian Journal of Botany
    Volume62
    Issue number5
    DOIs
    Publication statusPublished - 2014

    Fingerprint

    Radula
    revegetation
    Cyperaceae
    land restoration
    micropropagation
    tissue culture
    seed
    plant tissues
    seeds
    planting
    plant nurseries
    keystone species
    Middle East
    smoke
    pericarp
    seed quality
    kinetin
    soaking
    naphthaleneacetic acid
    benzyladenine

    Cite this

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    title = "Large-scale micropropagation of the Australian key species Gahnia radula (Cyperaceae) and its return to revegetation sites",
    abstract = "{\circledC} 2014 CSIRO. We report on the successful propagation of the sedge Gahnia radula (R.Br.) Benth. from seed by using plant tissue culture, and its successful establishment in the field. This keystone species, although common along parts of the eastern coast of Australia, is currently not available for revegetation because of a lack of efficient propagation methods, leading to the use of substitute species in many restoration programs. Even though seed quality is a common problem for G. radula, one population bearing filled seed was located in the near-east of Melbourne and after harvest of fruit in December 2011, seeds were successfully germinated in vitro after removal of the pericarp. Overnight soaking in sterile 10{\%} (v/v) smoke water before culturing enhanced in vitro germination from 29.2{\%} to 66.7{\%}. In vitro-grown seedlings were then used as starting material for tissue-culture propagation via shoot culture. A micropropagation rate of about six new plantlets per cycle was achieved within 5-6 weeks with liquid half-strength Murashige-Skoog medium and a pulse treatment with 10 M 6-benzylaminopurine (BAP) and 2 M naphthalene acetic acid (NAA). Plants rooted after receiving a pulse treatment with 5 M kinetin and were successfully acclimatised into potting mix and were ready for field planting after 5-6 months. Tube stock was planted into two field sites with minimal weed control. Survival was 98{\%} in both cases 1 month after planting and 54{\%} and 74{\%} after the summer. Division of in vitro-derived plants in the nursery was very successful, with 93-96{\%} establishment of divisions. This research highlights the important role of plant tissue culture in conserving biodiversity of native flora.",
    author = "A. Kodym and I. Clarke and C. Aponte and Shane Turner and Eric Bunn and J.C. Delpratt",
    year = "2014",
    doi = "10.1071/BT14091",
    language = "English",
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    pages = "417--427",
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    Large-scale micropropagation of the Australian key species Gahnia radula (Cyperaceae) and its return to revegetation sites. / Kodym, A.; Clarke, I.; Aponte, C.; Turner, Shane; Bunn, Eric; Delpratt, J.C.

    In: Australian Journal of Botany, Vol. 62, No. 5, 2014, p. 417-427.

    Research output: Contribution to journalArticle

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    AU - Kodym, A.

    AU - Clarke, I.

    AU - Aponte, C.

    AU - Turner, Shane

    AU - Bunn, Eric

    AU - Delpratt, J.C.

    PY - 2014

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    N2 - © 2014 CSIRO. We report on the successful propagation of the sedge Gahnia radula (R.Br.) Benth. from seed by using plant tissue culture, and its successful establishment in the field. This keystone species, although common along parts of the eastern coast of Australia, is currently not available for revegetation because of a lack of efficient propagation methods, leading to the use of substitute species in many restoration programs. Even though seed quality is a common problem for G. radula, one population bearing filled seed was located in the near-east of Melbourne and after harvest of fruit in December 2011, seeds were successfully germinated in vitro after removal of the pericarp. Overnight soaking in sterile 10% (v/v) smoke water before culturing enhanced in vitro germination from 29.2% to 66.7%. In vitro-grown seedlings were then used as starting material for tissue-culture propagation via shoot culture. A micropropagation rate of about six new plantlets per cycle was achieved within 5-6 weeks with liquid half-strength Murashige-Skoog medium and a pulse treatment with 10 M 6-benzylaminopurine (BAP) and 2 M naphthalene acetic acid (NAA). Plants rooted after receiving a pulse treatment with 5 M kinetin and were successfully acclimatised into potting mix and were ready for field planting after 5-6 months. Tube stock was planted into two field sites with minimal weed control. Survival was 98% in both cases 1 month after planting and 54% and 74% after the summer. Division of in vitro-derived plants in the nursery was very successful, with 93-96% establishment of divisions. This research highlights the important role of plant tissue culture in conserving biodiversity of native flora.

    AB - © 2014 CSIRO. We report on the successful propagation of the sedge Gahnia radula (R.Br.) Benth. from seed by using plant tissue culture, and its successful establishment in the field. This keystone species, although common along parts of the eastern coast of Australia, is currently not available for revegetation because of a lack of efficient propagation methods, leading to the use of substitute species in many restoration programs. Even though seed quality is a common problem for G. radula, one population bearing filled seed was located in the near-east of Melbourne and after harvest of fruit in December 2011, seeds were successfully germinated in vitro after removal of the pericarp. Overnight soaking in sterile 10% (v/v) smoke water before culturing enhanced in vitro germination from 29.2% to 66.7%. In vitro-grown seedlings were then used as starting material for tissue-culture propagation via shoot culture. A micropropagation rate of about six new plantlets per cycle was achieved within 5-6 weeks with liquid half-strength Murashige-Skoog medium and a pulse treatment with 10 M 6-benzylaminopurine (BAP) and 2 M naphthalene acetic acid (NAA). Plants rooted after receiving a pulse treatment with 5 M kinetin and were successfully acclimatised into potting mix and were ready for field planting after 5-6 months. Tube stock was planted into two field sites with minimal weed control. Survival was 98% in both cases 1 month after planting and 54% and 74% after the summer. Division of in vitro-derived plants in the nursery was very successful, with 93-96% establishment of divisions. This research highlights the important role of plant tissue culture in conserving biodiversity of native flora.

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    JF - Australian Journal of Botany

    SN - 0067-1924

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