Abstract
Materials and methods. The study was approved by the university animal ethics committee. Eight female pigs (Sus Scrofra, weighing 45-50 kg) were divided into 2 groups: group I included 4 animals that underwent laparoscopic kidney orthotopic transplantation on the left side. The right kidney was remained functional in situ. The pigs recovered and were observed for 1 week In the 4 hosts group II pigs underwent a laparoseopic kidney transplantation on the left side. With simultaneous clipping of the right ureter. After recovery, the pigs were observed for 4 weeks. A laparotomy for examination was performed prior to euthanasia.
Results. All 4 group I pigs survived for 1 week. The laparotomy showed normal graft perfusion with wall patent renal artery and vein as well as satisfactory urine output upon transection of ureter in 3 hosts. Renal artery stenosis occurred in one pig. In The Immediate kidney graft function was achieved in 3 group II pigs. The fourth died following extubation due to laryngospasm despite a functional graft. The average creatinine levels were 195.5 mu mol/L on day 3; 224.5 mu mol/L at week 1; 127 mu mol/L at week 2; 182.7 umol/L at week 3; and 154.7 umol/L at week 4.
Conclusion. Laparoscopic kidney transplantation was feasible and safe in a pig model with immediate graft function. This study will provide further evidence to support application of laparoscopic technique to human kidney transplant.
| Original language | English |
|---|---|
| Pages (from-to) | 1776-1779 |
| Number of pages | 4 |
| Journal | Transplantation Proceedings |
| Volume | 45 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Jun 2013 |
| Event | 24th International Congress of the Transplantation Society - Berlin, Germany Duration: 15 Jul 2012 → 19 Jul 2012 |
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Laparoscopic Kidney Orthotopic Transplant: Preclinical Study in the Pig Model. / He, B.; Musk, G. C.; Mou, L.; Waneck, G. L.; Delriviere, L.
In: Transplantation Proceedings, Vol. 45, No. 5, 06.2013, p. 1776-1779.Research output: Contribution to journal › Conference article
TY - JOUR
T1 - Laparoscopic Kidney Orthotopic Transplant: Preclinical Study in the Pig Model
AU - He, B.
AU - Musk, G. C.
AU - Mou, L.
AU - Waneck, G. L.
AU - Delriviere, L.
PY - 2013/6
Y1 - 2013/6
N2 - Background. Laparoscopic surgery has rapidly expanded in clinical practice replacing conventional open surgery over the last three decades. Laparoscopic donor nephrectomy has been favored due to its multiple benefits. The aim of this study was to explore the safety and feasibility of kidney transplantation by a laparoscopic technique in a pig model. Materials and methods. The study was approved by the university animal ethics committee. Eight female pigs (Sus Scrofra, weighing 45-50 kg) were divided into 2 groups: group I included 4 animals that underwent laparoscopic kidney orthotopic transplantation on the left side. The right kidney was remained functional in situ. The pigs recovered and were observed for 1 week In the 4 hosts group II pigs underwent a laparoseopic kidney transplantation on the left side. With simultaneous clipping of the right ureter. After recovery, the pigs were observed for 4 weeks. A laparotomy for examination was performed prior to euthanasia. Results. All 4 group I pigs survived for 1 week. The laparotomy showed normal graft perfusion with wall patent renal artery and vein as well as satisfactory urine output upon transection of ureter in 3 hosts. Renal artery stenosis occurred in one pig. In The Immediate kidney graft function was achieved in 3 group II pigs. The fourth died following extubation due to laryngospasm despite a functional graft. The average creatinine levels were 195.5 mu mol/L on day 3; 224.5 mu mol/L at week 1; 127 mu mol/L at week 2; 182.7 umol/L at week 3; and 154.7 umol/L at week 4. Conclusion. Laparoscopic kidney transplantation was feasible and safe in a pig model with immediate graft function. This study will provide further evidence to support application of laparoscopic technique to human kidney transplant.
AB - Background. Laparoscopic surgery has rapidly expanded in clinical practice replacing conventional open surgery over the last three decades. Laparoscopic donor nephrectomy has been favored due to its multiple benefits. The aim of this study was to explore the safety and feasibility of kidney transplantation by a laparoscopic technique in a pig model. Materials and methods. The study was approved by the university animal ethics committee. Eight female pigs (Sus Scrofra, weighing 45-50 kg) were divided into 2 groups: group I included 4 animals that underwent laparoscopic kidney orthotopic transplantation on the left side. The right kidney was remained functional in situ. The pigs recovered and were observed for 1 week In the 4 hosts group II pigs underwent a laparoseopic kidney transplantation on the left side. With simultaneous clipping of the right ureter. After recovery, the pigs were observed for 4 weeks. A laparotomy for examination was performed prior to euthanasia. Results. All 4 group I pigs survived for 1 week. The laparotomy showed normal graft perfusion with wall patent renal artery and vein as well as satisfactory urine output upon transection of ureter in 3 hosts. Renal artery stenosis occurred in one pig. In The Immediate kidney graft function was achieved in 3 group II pigs. The fourth died following extubation due to laryngospasm despite a functional graft. The average creatinine levels were 195.5 mu mol/L on day 3; 224.5 mu mol/L at week 1; 127 mu mol/L at week 2; 182.7 umol/L at week 3; and 154.7 umol/L at week 4. Conclusion. Laparoscopic kidney transplantation was feasible and safe in a pig model with immediate graft function. This study will provide further evidence to support application of laparoscopic technique to human kidney transplant.
U2 - 10.1016/j.transproceed.2013.01.056
DO - 10.1016/j.transproceed.2013.01.056
M3 - Conference article
VL - 45
SP - 1776
EP - 1779
JO - Transplantation Proceedings
JF - Transplantation Proceedings
SN - 0041-1345
IS - 5
ER -