Laminin γ3 plays an important role in retinal lamination, photoreceptor organisation and ganglion cell differentiation

Birthe Dorgau, Majed Felemban, Alexander Sharpe, Roman Bauer, Dean Hallam, David H. Steel, Susan Lindsay, Carla Mellough, Majlinda Lako

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Laminins are heterotrimeric glycoproteins of the extracellular matrix. Eleven different laminin chains have been identified in vertebrates. They are ubiquitously expressed in the human body, with a distinct tissue distribution. Laminin expression in neural retina and their functional role during human retinogenesis is still unknown. This study investigated the laminin expression in human developing and adult retina, showing laminin α1, α5, β1, β2 and γ1 to be predominantly expressed in Bruch's membrane and the inner limiting membrane. Laminin-332 and laminin γ3 expression were mainly observed in the neural retina during retinal histogenesis. These expression patterns were largely conserved in pluripotent stem cell-derived retinal organoids. Blocking of laminin γ3 function in retinal organoids resulted in the disruption of laminar organisation and synapse formation, the loss of photoreceptor organisation and retinal ganglion cells. Our data demonstrate a unique temporal and spatial expression for laminins and reveal a novel role for laminin γ3 during human retinogenesis.

Original languageEnglish
Article number648
JournalCell Death and Disease
Volume9
Issue number6
DOIs
Publication statusPublished - 1 Jun 2018

Fingerprint

Vertebrate Photoreceptor Cells
Laminin
Ganglia
Cell Differentiation
Organizations
Organoids
Retina
Bruch Membrane
Pluripotent Stem Cells
Retinal Ganglion Cells
Tissue Distribution
Human Body
Synapses
Extracellular Matrix
Vertebrates
Glycoproteins
Membranes

Cite this

Dorgau, Birthe ; Felemban, Majed ; Sharpe, Alexander ; Bauer, Roman ; Hallam, Dean ; Steel, David H. ; Lindsay, Susan ; Mellough, Carla ; Lako, Majlinda. / Laminin γ3 plays an important role in retinal lamination, photoreceptor organisation and ganglion cell differentiation. In: Cell Death and Disease. 2018 ; Vol. 9, No. 6.
@article{de384d8958de46b68510331a39aeb358,
title = "Laminin γ3 plays an important role in retinal lamination, photoreceptor organisation and ganglion cell differentiation",
abstract = "Laminins are heterotrimeric glycoproteins of the extracellular matrix. Eleven different laminin chains have been identified in vertebrates. They are ubiquitously expressed in the human body, with a distinct tissue distribution. Laminin expression in neural retina and their functional role during human retinogenesis is still unknown. This study investigated the laminin expression in human developing and adult retina, showing laminin α1, α5, β1, β2 and γ1 to be predominantly expressed in Bruch's membrane and the inner limiting membrane. Laminin-332 and laminin γ3 expression were mainly observed in the neural retina during retinal histogenesis. These expression patterns were largely conserved in pluripotent stem cell-derived retinal organoids. Blocking of laminin γ3 function in retinal organoids resulted in the disruption of laminar organisation and synapse formation, the loss of photoreceptor organisation and retinal ganglion cells. Our data demonstrate a unique temporal and spatial expression for laminins and reveal a novel role for laminin γ3 during human retinogenesis.",
author = "Birthe Dorgau and Majed Felemban and Alexander Sharpe and Roman Bauer and Dean Hallam and Steel, {David H.} and Susan Lindsay and Carla Mellough and Majlinda Lako",
year = "2018",
month = "6",
day = "1",
doi = "10.1038/s41419-018-0648-0",
language = "English",
volume = "9",
journal = "Cell Death & Disease",
issn = "2041-4889",
publisher = "Nature Publishing Group",
number = "6",

}

Laminin γ3 plays an important role in retinal lamination, photoreceptor organisation and ganglion cell differentiation. / Dorgau, Birthe; Felemban, Majed; Sharpe, Alexander; Bauer, Roman; Hallam, Dean; Steel, David H.; Lindsay, Susan; Mellough, Carla; Lako, Majlinda.

In: Cell Death and Disease, Vol. 9, No. 6, 648, 01.06.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Laminin γ3 plays an important role in retinal lamination, photoreceptor organisation and ganglion cell differentiation

AU - Dorgau, Birthe

AU - Felemban, Majed

AU - Sharpe, Alexander

AU - Bauer, Roman

AU - Hallam, Dean

AU - Steel, David H.

AU - Lindsay, Susan

AU - Mellough, Carla

AU - Lako, Majlinda

PY - 2018/6/1

Y1 - 2018/6/1

N2 - Laminins are heterotrimeric glycoproteins of the extracellular matrix. Eleven different laminin chains have been identified in vertebrates. They are ubiquitously expressed in the human body, with a distinct tissue distribution. Laminin expression in neural retina and their functional role during human retinogenesis is still unknown. This study investigated the laminin expression in human developing and adult retina, showing laminin α1, α5, β1, β2 and γ1 to be predominantly expressed in Bruch's membrane and the inner limiting membrane. Laminin-332 and laminin γ3 expression were mainly observed in the neural retina during retinal histogenesis. These expression patterns were largely conserved in pluripotent stem cell-derived retinal organoids. Blocking of laminin γ3 function in retinal organoids resulted in the disruption of laminar organisation and synapse formation, the loss of photoreceptor organisation and retinal ganglion cells. Our data demonstrate a unique temporal and spatial expression for laminins and reveal a novel role for laminin γ3 during human retinogenesis.

AB - Laminins are heterotrimeric glycoproteins of the extracellular matrix. Eleven different laminin chains have been identified in vertebrates. They are ubiquitously expressed in the human body, with a distinct tissue distribution. Laminin expression in neural retina and their functional role during human retinogenesis is still unknown. This study investigated the laminin expression in human developing and adult retina, showing laminin α1, α5, β1, β2 and γ1 to be predominantly expressed in Bruch's membrane and the inner limiting membrane. Laminin-332 and laminin γ3 expression were mainly observed in the neural retina during retinal histogenesis. These expression patterns were largely conserved in pluripotent stem cell-derived retinal organoids. Blocking of laminin γ3 function in retinal organoids resulted in the disruption of laminar organisation and synapse formation, the loss of photoreceptor organisation and retinal ganglion cells. Our data demonstrate a unique temporal and spatial expression for laminins and reveal a novel role for laminin γ3 during human retinogenesis.

UR - http://www.scopus.com/inward/record.url?scp=85047417900&partnerID=8YFLogxK

U2 - 10.1038/s41419-018-0648-0

DO - 10.1038/s41419-018-0648-0

M3 - Article

VL - 9

JO - Cell Death & Disease

JF - Cell Death & Disease

SN - 2041-4889

IS - 6

M1 - 648

ER -