Label-free volumetric imaging of conjunctival collecting lymphatics ex vivo by optical coherence tomography lymphangiography

Peijun Gong, Dao Yi Yu, Qiang Wang, Paula K. Yu, Karol Karnowski, Morgan Heisler, Ashley Francke, Dong An, Marinko V. Sarunic, David D. Sampson

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We employ optical coherence tomography (OCT) and optical coherence microscopy (OCM) to study conjunctival lymphatics in porcine eyes ex vivo. This study is a precursor to the development of in vivo imaging of the collecting lymphatics for potentially guiding and monitoring glaucoma filtration surgery. OCT scans at 1300 nm and higher-resolution OCM scans at 785 nm reveal the lymphatic vessels via their optical transparency. Equivalent signal characteristics are also observed from blood vessels largely free of blood (and devoid of flow) in the ex vivo conjunctiva. In our lymphangiography, vessel networks were segmented by compensating the depth attenuation in the volumetric OCT/OCM signal, projecting the minimum intensity in two dimensions and thresholding to generate a three-dimensional vessel volume. Vessel segmentation from multiple locations of a range of porcine eyes (n = 21) enables visualization of the vessel networks and indicates the varying spatial distribution of patent lymphatics. Such visualization provides a new tool to investigate conjunctival vessels in tissue ex vivo without need for histological tissue processing and a valuable reference on vessel morphology for the in vivo label-free imaging studies of lymphatics to follow.

Original languageEnglish
Article numbere201800070
JournalJournal of Biophotonics
Volume11
Issue number8
DOIs
Publication statusPublished - 1 Aug 2018

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Lymphography
Optical tomography
Optical Coherence Tomography
vessels
Labels
Microscopy
Microscopic examination
tomography
Imaging techniques
Swine
Visualization
Tissue
Filtering Surgery
Lymphatic Vessels
Conjunctiva
Blood vessels
Glaucoma
Transparency
Surgery
Spatial distribution

Cite this

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title = "Label-free volumetric imaging of conjunctival collecting lymphatics ex vivo by optical coherence tomography lymphangiography",
abstract = "We employ optical coherence tomography (OCT) and optical coherence microscopy (OCM) to study conjunctival lymphatics in porcine eyes ex vivo. This study is a precursor to the development of in vivo imaging of the collecting lymphatics for potentially guiding and monitoring glaucoma filtration surgery. OCT scans at 1300 nm and higher-resolution OCM scans at 785 nm reveal the lymphatic vessels via their optical transparency. Equivalent signal characteristics are also observed from blood vessels largely free of blood (and devoid of flow) in the ex vivo conjunctiva. In our lymphangiography, vessel networks were segmented by compensating the depth attenuation in the volumetric OCT/OCM signal, projecting the minimum intensity in two dimensions and thresholding to generate a three-dimensional vessel volume. Vessel segmentation from multiple locations of a range of porcine eyes (n = 21) enables visualization of the vessel networks and indicates the varying spatial distribution of patent lymphatics. Such visualization provides a new tool to investigate conjunctival vessels in tissue ex vivo without need for histological tissue processing and a valuable reference on vessel morphology for the in vivo label-free imaging studies of lymphatics to follow.",
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Label-free volumetric imaging of conjunctival collecting lymphatics ex vivo by optical coherence tomography lymphangiography. / Gong, Peijun; Yu, Dao Yi; Wang, Qiang; Yu, Paula K.; Karnowski, Karol; Heisler, Morgan; Francke, Ashley; An, Dong; Sarunic, Marinko V.; Sampson, David D.

In: Journal of Biophotonics, Vol. 11, No. 8, e201800070, 01.08.2018.

Research output: Contribution to journalArticle

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AU - Yu, Dao Yi

AU - Wang, Qiang

AU - Yu, Paula K.

AU - Karnowski, Karol

AU - Heisler, Morgan

AU - Francke, Ashley

AU - An, Dong

AU - Sarunic, Marinko V.

AU - Sampson, David D.

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