Projects per year
Abstract
Metabolism, auxin signaling and reactive oxygen species (ROS) all contribute to plant growth, and each is linked to plant mitochondria and the process of respiration. Knockdown of mitochondrial succinate dehydrogenase assembly factor 2 (SDHAF2) in Arabidopsis thaliana lowered succinate dehydrogenase activity and led to pH-inducible root inhibition when the growth medium pH was poised at different points between 7.0 and 5.0, but this phenomenon was not observed in wildtype (WT). Roots of sdhaf2 mutants showed high accumulation of succinate, depletion of citrate and malate and up-regulation of ROS-related and stress-inducible genes at pH 5.5. A change of oxidative status in sdhaf2 roots at low pH was also evidenced by low ROS staining in root tips and altered root sensitivity to H2O2. sdhaf2 had low auxin activity in root tips via DR5-GUS staining but displayed increased indole-3-acetic acid (IAA, auxin) abundance and IAA hypersensitivity, which is most likely caused by the change in ROS levels. On this basis, we conclude that knockdown of SDHAF2 induces pH-related root elongation and auxin hyperaccumulation and hypersensitivity, mediated by altered ROS homeostasis. This observation extends the existing evidence of associations between mitochondrial function and auxin by establishing a cascade of cellular events that link them through ROS formation, metabolism and root growth at different pH values.
Original language | English |
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Pages (from-to) | 1185-1198 |
Number of pages | 14 |
Journal | Plant & Cell Physiology |
Volume | 62 |
Issue number | 7 |
Early online date | 26 May 2021 |
DOIs | |
Publication status | Published - 1 Jul 2021 |
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Dive into the research topics of 'Knockdown of Succinate Dehydrogenase Assembly Factor 2 Induces Reactive Oxygen Species-Mediated Auxin Hypersensitivity Causing pH-Dependent Root Elongation'. Together they form a unique fingerprint.Projects
- 3 Finished
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Defining factors in the control of protein turnover in plants
Millar, H. (Investigator 01)
ARC Australian Research Council
1/01/18 → 31/03/21
Project: Research
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ARC Centre of Excellence in Plant Energy Biology 2014 (CPEB2)
Millar, H. (Investigator 01), Pogson, B. (Investigator 02), Tyerman, S. (Investigator 03), Small, I. (Investigator 04), Whelan, J. (Investigator 05), Borevitz, J. (Investigator 06), Lister, R. (Investigator 07), Atkin, O. (Investigator 08) & Munns, R. (Investigator 09)
ARC Australian Research Council
1/01/14 → 31/05/21
Project: Research
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Dissecting novel roles of succinate dehydrogenase in stomatal aperture and root elongation in plants
Huang, S. (Investigator 01)
ARC Australian Research Council
1/01/13 → 31/12/18
Project: Research