WUSCHEL-related homeobox family genes in rice control lateral root primordium size

Tsubasa Kawai, Kyosuke Shibata, Ryosuke Akahoshi, Shunsaku Nishiuchi, Hirokazu Takahashi, Mikio Nakazono, Takaaki Kojima, Misuzu Nosaka-Takahashi, Yutaka Sato, Atsushi Toyoda, Nonawin Lucob-Agustin, Mana Kano-Nakata, Roel R Suralta, Jonathan M Niones, Yinglong Chen, Kadambot H M Siddique, Akira Yamauchi, Yoshiaki Inukai

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

The development of a plastic root system is essential for stable crop production under variable environments. Rice plants have two types of lateral roots (LRs): S-type (short and thin) and L-type (long, thick, and capable of further branching). LR types are determined at the primordium stage, with a larger primordium size in L-types than S-types. Despite the importance of LR types for rice adaptability to variable water conditions, molecular mechanisms underlying the primordium size control of LRs are unknown. Here, we show that two WUSCHEL-related homeobox (WOX) genes have opposing roles in controlling LR primordium (LRP) size in rice. Root tip excision on seminal roots induced L-type LR formation with wider primordia formed from an early developmental stage. QHB/OsWOX5 was isolated as a causative gene of a mutant that is defective in S-type LR formation but produces more L-type LRs than wild-type (WT) plants following root tip excision. A transcriptome analysis revealed that OsWOX10 is highly up-regulated in L-type LRPs. OsWOX10 overexpression in LRPs increased the LR diameter in an expression-dependent manner. Conversely, the mutation in OsWOX10 decreased the L-type LR diameter under mild drought conditions. The qhb mutants had higher OsWOX10 expression than WT after root tip excision. A yeast one-hybrid assay revealed that the transcriptional repressive activity of QHB was lost in qhb mutants. An electrophoresis mobility shift assay revealed that OsWOX10 is a potential target of QHB. These data suggest that QHB represses LR diameter increase, repressing OsWOX10 Our findings could help improve root system plasticity under variable environments.

Original languageEnglish
Article numbere2101846119
Number of pages9
JournalProceedings of the National Academy of Sciences of the United States of America
Volume119
Issue number1
DOIs
Publication statusPublished - 4 Jan 2022

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