@inbook{eefcfcd5249b438b9056518fe6f9b611,
title = "Isolation of Plant Mitochondria Using Affinity Purification",
abstract = "Mitochondria are highly dynamic organelles that are vital for eukaryotic cellular metabolism. Mitochondria account only for a small fraction of the total cell volume. As such, intact organelle isolation is a basic requirement to study mitochondrial biochemistry and physiology. However, established isolation procedures are tedious, and require a substantial amount of tissue material to start with. We addressed this matter and developed a single step purification for Arabidopsis thaliana (Arabidopsis) mitochondria. Ubiquitous expression of an 3xHA-sGFP-TOM5 fusion protein in the outer mitochondrial membrane allows affinity purification by immunocapture in a single step. We here provide a detailed step-by-step protocol for rapid immunocapture of intact and physiologically active mitochondria from Arabidopsis plants.",
keywords = "Arabidopsis, HA-tag, Immunocapture, Magnetic beads, Mitochondria, Organelle isolation, TOM5",
author = "Franziska Kuhnert and Weber, {Andreas P.M.}",
year = "2022",
doi = "10.1007/978-1-0716-1653-6_3",
language = "English",
isbn = "978-1-0716-1652-9",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "25--37",
editor = "Aken, {Olivier Van } and Rasmusson, {Allan G. }",
booktitle = "Plant Mitochondria",
address = "United States",
}