Isolation of mitochondria, their sub-organellar compartments, and membranes

Research output: Chapter in Book/Conference paperChapter

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Abstract

Mitochondria are the sites of a diverse set of essential biochemical processes in plants. In order to facilitate the analysis of these functions, this chapter presents protocols for the isolation of intact mitochondria from a range of plant tissues as well two workfl ows for fractionation into their four subcompartments; the inner and outer membranes and the two aqueous compartments, the inter membrane space and matrix. Protocols for the assessment of mitochondrial integrity and purity through enzymatic function and suggestions of commercially available compartment marker antibodies are provided.

Original languageEnglish
Title of host publicationIsolation of Plant Organelles and Structures
Subtitle of host publicationMethods and Protocols
PublisherHumana Press
Pages83-96
Number of pages14
Volume1511
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1511
ISSN (Print)10643745

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  • Cite this

    Duncan, O., Harvey Millar, A., & Taylor, N. L. (2016). Isolation of mitochondria, their sub-organellar compartments, and membranes. In Isolation of Plant Organelles and Structures: Methods and Protocols (Vol. 1511, pp. 83-96). (Methods in Molecular Biology; Vol. 1511). Humana Press. https://doi.org/10.1007/978-1-4939-6533-5_7