Isolation of mitochondria from model and crop plants

Sandra M. Kerbler, Nicolas L. Taylor

Research output: Chapter in Book/Conference paperOther chapter contribution

1 Citation (Scopus)

Abstract

The ability to isolate intact and functional mitochondria has greatly deepened our understanding of mitochondrial structure and function. With the advancement of molecular biology techniques and progression into omics-based research over recent decades, mitochondrial research has shifted from crop species such as wheat, pea, and potato to genetically sequenced models such as Arabidopsis thaliana and rice. Although there are many attributes that make model species particularly appealing for plant research, they are often less than ideal for conducting biochemical investigations and as such, considerable modification to mitochondrial isolation methods has been made. As the cost of genome sequencing continues to decrease however, an increasing number of crop species are now being sequenced and with these new resources it appears that the research community is turning back toward crop research. In this chapter we present mitochondrial isolation methods using density gradient centrifugation for both model species such as Arabidopsis thaliana, rice, and Medicago and crop species including wheat, potato, and pea. In addition, we present a number of marker enzyme assays to confirm mitochondrial purity as well as respiratory assays to determine outer membrane integrity and respiratory function of isolated mitochondria.

Original languageEnglish
Title of host publicationPlant respiration and internal oxygen
Subtitle of host publicationMethods and protocols
EditorsKapuganti Jagadis Gupta
Place of PublicationNew York
PublisherHumana Press
Chapter12
Pages115-142
Number of pages28
ISBN (Electronic)9781493972920
ISBN (Print)9781493972913
DOIs
Publication statusPublished - 5 Sep 2017

Publication series

NameMethods in Molecular Biology
Volume1670
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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