Isolation of Intact Mitochondria from the Model Plant Species "Arabidopsis thaliana" and "Oryza sativa"

Monika Murcha, James Whelan

Research output: Chapter in Book/Conference paperChapterpeer-review

41 Citations (Scopus)

Abstract

The study of mitochondrial composition, biogenesis, and metabolism requires the isolation of intact functional mitochondria, often in relatively large quantities sufficient for downstream biochemical and proteomic analysis. Here we describe techniques optimized for the isolation of functional mitochondria from a variety of tissues from the model monocot and dicot species Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa).
Original languageEnglish
Title of host publicationPlant Mitochondria: methods and protocols
Editors Whelan, J. Murcha, M
Place of PublicationUSA
PublisherHumana Press Inc.
Pages1-12
Volume1305
ISBN (Print)9781493926398
DOIs
Publication statusPublished - 2015

Publication series

NameMethods in Molecular Biology

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