TY - JOUR
T1 - Isolation and Sequencing of Chromosome Arm 7RS of Rye, Secale cereale
AU - Petereit, Jakob
AU - Tay Fernandez, Cassandria
AU - Marsh, Jacob I.
AU - Bayer, Philipp E.
AU - Thomas, William J.W.
AU - Aliyeva, Aybeniz Javad
AU - Karafiátová, Miroslava
AU - Doležel, Jaroslav
AU - Batley, Jacqueline
AU - Edwards, David
N1 - Funding Information:
This work is funded by the Australia Research Council (Projects DP210100296, DP200100762, and DE210100398). This work was supported by resources provided by the Pawsey Supercomputing Centre with funding from the Australian Government and the Government of Western Australia. M.K. and J.D. were supported by the ERDF project “Plants as a tool for sustainable global development” (No. CZ.02.1.01/0.0/0.0/16_019/0000827). W.J.W.T. receives supports from the Grains Research and Development Corporation.
Publisher Copyright:
© 2022 by the authors.
PY - 2022/10
Y1 - 2022/10
N2 - Rye (Secale cereale) is a climate-resilient cereal grown extensively as grain or forage crop in Northern and Eastern Europe. In addition to being an important crop, it has been used to improve wheat through introgression of genomic regions for improved yield and disease resistance. Understanding the genomic diversity of rye will assist both the improvement of this crop and facilitate the introgression of more valuable traits into wheat. Here, we isolated and sequenced the short arm of rye chromosome 7 (7RS) from Triticale 380SD using flow cytometry and compared it to the public Lo7 rye whole genome reference assembly. We identify 2747 Lo7 genes present on the isolated chromosome arm and two clusters containing seven and sixty-five genes that are present on Triticale 380SD 7RS, but absent from Lo7 7RS. We identified 29 genes that are not assigned to chromosomal locations in the Lo7 assembly but are present on Triticale 380SD 7RS, suggesting a chromosome arm location for these genes. Our study supports the Lo7 reference assembly and provides a repertoire of genes on Triticale 7RS.
AB - Rye (Secale cereale) is a climate-resilient cereal grown extensively as grain or forage crop in Northern and Eastern Europe. In addition to being an important crop, it has been used to improve wheat through introgression of genomic regions for improved yield and disease resistance. Understanding the genomic diversity of rye will assist both the improvement of this crop and facilitate the introgression of more valuable traits into wheat. Here, we isolated and sequenced the short arm of rye chromosome 7 (7RS) from Triticale 380SD using flow cytometry and compared it to the public Lo7 rye whole genome reference assembly. We identify 2747 Lo7 genes present on the isolated chromosome arm and two clusters containing seven and sixty-five genes that are present on Triticale 380SD 7RS, but absent from Lo7 7RS. We identified 29 genes that are not assigned to chromosomal locations in the Lo7 assembly but are present on Triticale 380SD 7RS, suggesting a chromosome arm location for these genes. Our study supports the Lo7 reference assembly and provides a repertoire of genes on Triticale 7RS.
KW - isolated chromosome arm sequencing
KW - presence-absence variation
KW - rye
UR - http://www.scopus.com/inward/record.url?scp=85139904526&partnerID=8YFLogxK
U2 - 10.3390/ijms231911106
DO - 10.3390/ijms231911106
M3 - Article
C2 - 36232406
AN - SCOPUS:85139904526
SN - 1661-6596
VL - 23
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 19
M1 - 11106
ER -