Isolation and characterization of temperate Bacteriophages of Clostridium difficile

S. Goh; Thomas Riley; Barbara Chang

doi:10.1128/AEM.71.2.1079-1083.2005

Isolation and characterization of temperate Bacteriophages of Clostridium difficile

S. Goh, Thomas Riley, Barbara Chang

Research output: Contribution to journal › Article › peer-review

58 Citations (Scopus)

Abstract

The lack of information on bacteriophages of Clostridium difficile prompted this study. Three of 56 clinical C. difficile isolates yielded double-stranded DNA phages phiC2, phiC5, phiC6, and phiC8 upon induction. Superinfection and DNA analyses revealed relatedness between the phages, while partial sequencing of phiC2 showed nucleotide homology to the sequenced C. difficile strain CD630.

Original language	English
Pages (from-to)	1079-1083
Journal	Applied and Environmental Microbiology
Volume	71
Issue number	2
DOIs	https://doi.org/10.1128/AEM.71.2.1079-1083.2005
Publication status	Published - 2005

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10.1128/AEM.71.2.1079-1083.2005

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title = "Isolation and characterization of temperate Bacteriophages of Clostridium difficile",

abstract = "The lack of information on bacteriophages of Clostridium difficile prompted this study. Three of 56 clinical C. difficile isolates yielded double-stranded DNA phages phiC2, phiC5, phiC6, and phiC8 upon induction. Superinfection and DNA analyses revealed relatedness between the phages, while partial sequencing of phiC2 showed nucleotide homology to the sequenced C. difficile strain CD630.",

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AB - The lack of information on bacteriophages of Clostridium difficile prompted this study. Three of 56 clinical C. difficile isolates yielded double-stranded DNA phages phiC2, phiC5, phiC6, and phiC8 upon induction. Superinfection and DNA analyses revealed relatedness between the phages, while partial sequencing of phiC2 showed nucleotide homology to the sequenced C. difficile strain CD630.

U2 - 10.1128/AEM.71.2.1079-1083.2005

DO - 10.1128/AEM.71.2.1079-1083.2005

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JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

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Isolation and characterization of temperate Bacteriophages of Clostridium difficile

Abstract

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