Isolation and Characterization of Arbuscules from Roots of an Increased-arbuscule-forming Mutant of Lotus japonicus

K. Senoo, Zakaria Solaiman, S. Tanaka, M. Kawaguchi, H. Imaizumi-Anraku, S. Akao, A. Tanaka, H. Obata

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    Abstract

    Background and Aims Previous methods for isolation of arbuscules from mycorrhizal roots are time-consuming, complex and expensive. Therefore, a simple, rapid and inexpensive method for the isolation of metabolically active arbuscules from plant root of an increased-arbuscule-forming mutant of Lotus japonicus (Ljsym78-2) is described.Methods Roots of the L. japonicus mutant plants Ljsym78-2 colonized by Glomus sp. were separated from soil, washed with water, immersed in CaSO4 before being cut into 5-mm pieces and homogenized with a Waring blender at 6000 rpm for 30 s. The arbuscules were purified by separation from plant tissues with a 50-mu m nylon mesh, finally collecting on a 30-mu m nylon mesh. Enzyme histochemical staining showed that the collected arbuscules had succinate dehydrogenase, alkaline phosphatase and acid phosphatase activities.Key Results and Conclusions The enzymic activity of the arbuscules was not affected after the isolation process. The establishment of this simple, rapid and inexpensive method for the isolation of metabolically active arbuscules will be useful to clarify the biochemical processes occurring in nutrient exchange at the arbuscular interface.
    Original languageEnglish
    Pages (from-to)1599-1603
    JournalAnnals of Botany
    Volume100
    DOIs
    Publication statusPublished - 2007

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