Isolation and characterization of 13 polymorphic microsatellite loci for the smooth Cherax cainii and hairy marron C. tenuimanus (Decapoda: Parastacidae)

Jason Kennington, C. Guildea, Sherralee Lukehurst, Y. Hitchen, M. Gardner, R. Duffy, Joana Dias, Jason Ledger, M. Snow

Research output: Contribution to journalLetter

2 Citations (Scopus)

Abstract

Numbers of the critically endangered hairy marron Cherex tenuimanus are declining rapidly in its natural habitat, mainly due to competition with smooth marron C. cainii. To determine whether genetic introgression is occurring between the two species we isolated 13 new polymorphic microsatellite markers from C. cainii using 454 shotgun sequencing. The loci were screened for variation in 20 individuals of C. cainii and eight individuals of C. tenuimanus. There was no evidence of linkage disequilibrium between pairs of loci and only one locus deviated significantly from Hardy-Weinberg equilibrium. The number of alleles per locus ranged from two to 11 across both species. © 2013 Springer Science+Business Media Dordrecht.
Original languageEnglish
Pages (from-to)337-339
JournalConservation Genetics Resources
Volume6
Issue number2
DOIs
Publication statusPublished - 2014

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Cherax tenuimanus
Cherax
Parastacidae
Linkage Disequilibrium
Firearms
Decapoda
Microsatellite Repeats
Ecosystem
Alleles
microsatellite repeats
loci
introgression
disequilibrium
allele
habitat
linkage disequilibrium
sequence analysis
alleles
habitats

Cite this

@article{b99a1eed5284493d925ae843882b01e5,
title = "Isolation and characterization of 13 polymorphic microsatellite loci for the smooth Cherax cainii and hairy marron C. tenuimanus (Decapoda: Parastacidae)",
abstract = "Numbers of the critically endangered hairy marron Cherex tenuimanus are declining rapidly in its natural habitat, mainly due to competition with smooth marron C. cainii. To determine whether genetic introgression is occurring between the two species we isolated 13 new polymorphic microsatellite markers from C. cainii using 454 shotgun sequencing. The loci were screened for variation in 20 individuals of C. cainii and eight individuals of C. tenuimanus. There was no evidence of linkage disequilibrium between pairs of loci and only one locus deviated significantly from Hardy-Weinberg equilibrium. The number of alleles per locus ranged from two to 11 across both species. {\circledC} 2013 Springer Science+Business Media Dordrecht.",
author = "Jason Kennington and C. Guildea and Sherralee Lukehurst and Y. Hitchen and M. Gardner and R. Duffy and Joana Dias and Jason Ledger and M. Snow",
year = "2014",
doi = "10.1007/s12686-013-0088-1",
language = "English",
volume = "6",
pages = "337--339",
journal = "Conservation Genetics Resources",
issn = "1877-7252",
publisher = "Springer-Verlag London Ltd.",
number = "2",

}

Isolation and characterization of 13 polymorphic microsatellite loci for the smooth Cherax cainii and hairy marron C. tenuimanus (Decapoda: Parastacidae). / Kennington, Jason; Guildea, C.; Lukehurst, Sherralee; Hitchen, Y.; Gardner, M.; Duffy, R.; Dias, Joana; Ledger, Jason; Snow, M.

In: Conservation Genetics Resources, Vol. 6, No. 2, 2014, p. 337-339.

Research output: Contribution to journalLetter

TY - JOUR

T1 - Isolation and characterization of 13 polymorphic microsatellite loci for the smooth Cherax cainii and hairy marron C. tenuimanus (Decapoda: Parastacidae)

AU - Kennington, Jason

AU - Guildea, C.

AU - Lukehurst, Sherralee

AU - Hitchen, Y.

AU - Gardner, M.

AU - Duffy, R.

AU - Dias, Joana

AU - Ledger, Jason

AU - Snow, M.

PY - 2014

Y1 - 2014

N2 - Numbers of the critically endangered hairy marron Cherex tenuimanus are declining rapidly in its natural habitat, mainly due to competition with smooth marron C. cainii. To determine whether genetic introgression is occurring between the two species we isolated 13 new polymorphic microsatellite markers from C. cainii using 454 shotgun sequencing. The loci were screened for variation in 20 individuals of C. cainii and eight individuals of C. tenuimanus. There was no evidence of linkage disequilibrium between pairs of loci and only one locus deviated significantly from Hardy-Weinberg equilibrium. The number of alleles per locus ranged from two to 11 across both species. © 2013 Springer Science+Business Media Dordrecht.

AB - Numbers of the critically endangered hairy marron Cherex tenuimanus are declining rapidly in its natural habitat, mainly due to competition with smooth marron C. cainii. To determine whether genetic introgression is occurring between the two species we isolated 13 new polymorphic microsatellite markers from C. cainii using 454 shotgun sequencing. The loci were screened for variation in 20 individuals of C. cainii and eight individuals of C. tenuimanus. There was no evidence of linkage disequilibrium between pairs of loci and only one locus deviated significantly from Hardy-Weinberg equilibrium. The number of alleles per locus ranged from two to 11 across both species. © 2013 Springer Science+Business Media Dordrecht.

U2 - 10.1007/s12686-013-0088-1

DO - 10.1007/s12686-013-0088-1

M3 - Letter

VL - 6

SP - 337

EP - 339

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

SN - 1877-7252

IS - 2

ER -