Fourteen nuclear microsatellite markers were developed for the short-range endemic tree, Banksia arborea to examine patterns of genetic diversity and structure across the species' restricted distribution. One hexanucleotide, three trinucleotide and 10 dinucleotide repeat loci were developed and tested on 24 individuals from one population. The number of alleles per locus ranged from 2 to 11. Observed and expected heterozygosity ranged from 0 to 0.833 and 0.044 to 0.823 respectively. All loci showed independent inheritance and null alleles are suspected to occur in four loci. © 2013 Springer Science+Business Media Dordrecht.