Isolation and characterisation of 11 microsatellite loci from the Western Australian Spirostreptid millipede, Atelomastix bamfordi

Nuclear microsatellite markers were developed for the Western Australian, short-range endemic millipede Atelomastix bamfordi to study patterns of population genetic structure across the species' terrestrial island-like distribution. Five dinucleotide, one trinucleotide, four tetranucleotide and one pentanucleotide repeat loci were developed and tested on 22 individuals sampled from one population. The number of alleles per locus ranged from 2 to 11 and observed heterozygosity ranged from 0. 091 to 0. 773. Null alleles were suspected to occur at four loci, but all 11 loci showed independent inheritance. Four loci were useful in cross-amplification in another Atelomastix species. © 2012 Springer Science+Business Media Dordrecht.