Investigation of the role of epigenetic modification in keloid scar pathology

Mansour Abdullah S Alghamdi

    Research output: ThesisDoctoral Thesis

    Abstract

    Keloid scarring is a fibro-proliferative disorder of the skin with no effective treatment and poorly understood pathophysiology. This study investigated epigenetic modification and epithelial-mesenchymal interaction as possible molecular mechanisms important in keloid scarring. Genome-wide DNA methylation and transcriptome analysis was conducted using keloid fibroblasts from 12 patients. An integrated list of differentially methylated and expressed genes was generated. This may represent genes important in keloid pathology. Keratinocyte-fibroblast co-culture experiments showed changes in collagen production in both keloid and normal skin fibroblasts in response to keloid keratinocytes. This suggests epithelial-mesenchymal cross-talk also plays a role in keloid scarring.
    LanguageEnglish
    QualificationDoctor of Philosophy
    Awarding Institution
    • The University of Western Australia
    Award date25 Oct 2016
    StateUnpublished - 2016

    Fingerprint

    Keloid
    Epigenomics
    Cicatrix
    Pathology
    Fibroblasts
    Keratinocytes
    Skin
    Gene Expression Profiling
    DNA Methylation
    Coculture Techniques
    Genes
    Collagen
    Genome

    Cite this

    @phdthesis{a942e64464974b49bafac97d72f1c2da,
    title = "Investigation of the role of epigenetic modification in keloid scar pathology",
    abstract = "Keloid scarring is a fibro-proliferative disorder of the skin with no effective treatment and poorly understood pathophysiology. This study investigated epigenetic modification and epithelial-mesenchymal interaction as possible molecular mechanisms important in keloid scarring. Genome-wide DNA methylation and transcriptome analysis was conducted using keloid fibroblasts from 12 patients. An integrated list of differentially methylated and expressed genes was generated. This may represent genes important in keloid pathology. Keratinocyte-fibroblast co-culture experiments showed changes in collagen production in both keloid and normal skin fibroblasts in response to keloid keratinocytes. This suggests epithelial-mesenchymal cross-talk also plays a role in keloid scarring.",
    keywords = "Keloid scarring, Epigenetic, DNA methylation , Gene expression, Epithelial-mesenchymal interaction, Collagen tyoe I, Fibroblast , Keratinocyte",
    author = "Alghamdi, {Mansour Abdullah S}",
    year = "2016",
    language = "English",
    school = "The University of Western Australia",

    }

    Alghamdi, MAS 2016, 'Investigation of the role of epigenetic modification in keloid scar pathology', Doctor of Philosophy, The University of Western Australia.

    Investigation of the role of epigenetic modification in keloid scar pathology. / Alghamdi, Mansour Abdullah S.

    2016.

    Research output: ThesisDoctoral Thesis

    TY - THES

    T1 - Investigation of the role of epigenetic modification in keloid scar pathology

    AU - Alghamdi,Mansour Abdullah S

    PY - 2016

    Y1 - 2016

    N2 - Keloid scarring is a fibro-proliferative disorder of the skin with no effective treatment and poorly understood pathophysiology. This study investigated epigenetic modification and epithelial-mesenchymal interaction as possible molecular mechanisms important in keloid scarring. Genome-wide DNA methylation and transcriptome analysis was conducted using keloid fibroblasts from 12 patients. An integrated list of differentially methylated and expressed genes was generated. This may represent genes important in keloid pathology. Keratinocyte-fibroblast co-culture experiments showed changes in collagen production in both keloid and normal skin fibroblasts in response to keloid keratinocytes. This suggests epithelial-mesenchymal cross-talk also plays a role in keloid scarring.

    AB - Keloid scarring is a fibro-proliferative disorder of the skin with no effective treatment and poorly understood pathophysiology. This study investigated epigenetic modification and epithelial-mesenchymal interaction as possible molecular mechanisms important in keloid scarring. Genome-wide DNA methylation and transcriptome analysis was conducted using keloid fibroblasts from 12 patients. An integrated list of differentially methylated and expressed genes was generated. This may represent genes important in keloid pathology. Keratinocyte-fibroblast co-culture experiments showed changes in collagen production in both keloid and normal skin fibroblasts in response to keloid keratinocytes. This suggests epithelial-mesenchymal cross-talk also plays a role in keloid scarring.

    KW - Keloid scarring

    KW - Epigenetic

    KW - DNA methylation

    KW - Gene expression

    KW - Epithelial-mesenchymal interaction

    KW - Collagen tyoe I

    KW - Fibroblast

    KW - Keratinocyte

    M3 - Doctoral Thesis

    ER -