Investigation of the interaction between the protein components of human mitochondrial RNAse P

Lachlan Wheeler

doi:10.26182/2hws-de50

Investigation of the interaction between the protein components of human mitochondrial RNAse P

Lachlan Wheeler

UWA Medical School

Research output: Thesis › Master's Thesis

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Abstract

Human mitochondrial RNAse P is a protein complex containing three proteins MRPP1, MRPP2 and MRPP3. It is different to most RNAse P proteins due to the lack of an RNA component. While the structures of MRPP2 and MRPP3 are known, the structure of MRPP1 and the mechanism of interaction between the subunits is unknown. This study used a yeast-based system to investigate the interactions between these proteins via mutagenic screening. An area on MRPP1 was identified as a potential interaction site for MRPP2. For MRPP2, a candidate residue was identified as potentially being key for its interaction with MRPP1.

Original language	English
Qualification	Masters
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Filipovska, Aleksandra, Supervisor Rackham, Oliver, Supervisor
Award date	7 Oct 2022
DOIs	https://doi.org/10.26182/2hws-de50
Publication status	Unpublished - 2022

Embargo information

Embargoed from 11/10/2022 to 22/08/2024. Made publicly available on 22/08/2024.

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10.26182/2hws-de50

THESIS - MASTER OF PHILOSOPHY - WHEELER Lachlan Phillip Gordon - 2022
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Cite this

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title = "Investigation of the interaction between the protein components of human mitochondrial RNAse P",

abstract = "Human mitochondrial RNAse P is a protein complex containing three proteins MRPP1, MRPP2 and MRPP3. It is different to most RNAse P proteins due to the lack of an RNA component. While the structures of MRPP2 and MRPP3 are known, the structure of MRPP1 and the mechanism of interaction between the subunits is unknown. This study used a yeast-based system to investigate the interactions between these proteins via mutagenic screening. An area on MRPP1 was identified as a potential interaction site for MRPP2. For MRPP2, a candidate residue was identified as potentially being key for its interaction with MRPP1. ",

keywords = "mitochondria, RNAse P, MRPP1, MRPP2, mutagenesis, protein interaction, yeast two-hybrid",

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year = "2022",

doi = "10.26182/2hws-de50",

language = "English",

school = "The University of Western Australia",

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T1 - Investigation of the interaction between the protein components of human mitochondrial RNAse P

AU - Wheeler, Lachlan

PY - 2022

Y1 - 2022

N2 - Human mitochondrial RNAse P is a protein complex containing three proteins MRPP1, MRPP2 and MRPP3. It is different to most RNAse P proteins due to the lack of an RNA component. While the structures of MRPP2 and MRPP3 are known, the structure of MRPP1 and the mechanism of interaction between the subunits is unknown. This study used a yeast-based system to investigate the interactions between these proteins via mutagenic screening. An area on MRPP1 was identified as a potential interaction site for MRPP2. For MRPP2, a candidate residue was identified as potentially being key for its interaction with MRPP1.

AB - Human mitochondrial RNAse P is a protein complex containing three proteins MRPP1, MRPP2 and MRPP3. It is different to most RNAse P proteins due to the lack of an RNA component. While the structures of MRPP2 and MRPP3 are known, the structure of MRPP1 and the mechanism of interaction between the subunits is unknown. This study used a yeast-based system to investigate the interactions between these proteins via mutagenic screening. An area on MRPP1 was identified as a potential interaction site for MRPP2. For MRPP2, a candidate residue was identified as potentially being key for its interaction with MRPP1.

KW - mitochondria

KW - RNAse P

KW - MRPP1

KW - MRPP2

KW - mutagenesis

KW - protein interaction

KW - yeast two-hybrid

U2 - 10.26182/2hws-de50

DO - 10.26182/2hws-de50

M3 - Master's Thesis

ER -

Investigation of the interaction between the protein components of human mitochondrial RNAse P

Abstract

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