Investigating the role of epigenetics in scar maintenance

Andrew William Stevenson

Investigating the role of epigenetics in scar maintenance

Research output: Thesis › Doctoral Thesis

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Abstract

Scar is defined by an aberrant dermal matrix containing excess and densely packed collagen .This study aimed to characterise DNA methylation and gene expression patterns that underlie the production of collagen by scar fibroblasts. Dermal fibroblasts from normal skin and scar were cultured in vitro and profiled using methylation and expression arrays. Bioinformatic analysis identified a set of genes both differentially methylated and expressed in scar fibroblasts compared to normal skin fibroblasts .Modulation of expression of two identified genes altered collagen metabolism. This work suggests scar fibroblasts have a distinct methylome and transcriptome that may underpin aberrant collagen production.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Award date	6 Dec 2016
Publication status	Unpublished - 2016

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THESIS - DOCTOR OF PHILOSOPHY - STEVENSON Andrew William - 2016
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title = "Investigating the role of epigenetics in scar maintenance",

abstract = "Scar is defined by an aberrant dermal matrix containing excess and densely packed collagen .This study aimed to characterise DNA methylation and gene expression patterns that underlie the production of collagen by scar fibroblasts. Dermal fibroblasts from normal skin and scar were cultured in vitro and profiled using methylation and expression arrays. Bioinformatic analysis identified a set of genes both differentially methylated and expressed in scar fibroblasts compared to normal skin fibroblasts .Modulation of expression of two identified genes altered collagen metabolism. This work suggests scar fibroblasts have a distinct methylome and transcriptome that may underpin aberrant collagen production.",

keywords = "DNA methylation, Scarring, Fibrosis, Dermal matrix, Extracellular matrix, Scar skin, methylome, Transcriptome",

author = "Stevenson, {Andrew William}",

year = "2016",

language = "English",

school = "The University of Western Australia",

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T1 - Investigating the role of epigenetics in scar maintenance

AU - Stevenson, Andrew William

PY - 2016

Y1 - 2016

N2 - Scar is defined by an aberrant dermal matrix containing excess and densely packed collagen .This study aimed to characterise DNA methylation and gene expression patterns that underlie the production of collagen by scar fibroblasts. Dermal fibroblasts from normal skin and scar were cultured in vitro and profiled using methylation and expression arrays. Bioinformatic analysis identified a set of genes both differentially methylated and expressed in scar fibroblasts compared to normal skin fibroblasts .Modulation of expression of two identified genes altered collagen metabolism. This work suggests scar fibroblasts have a distinct methylome and transcriptome that may underpin aberrant collagen production.

AB - Scar is defined by an aberrant dermal matrix containing excess and densely packed collagen .This study aimed to characterise DNA methylation and gene expression patterns that underlie the production of collagen by scar fibroblasts. Dermal fibroblasts from normal skin and scar were cultured in vitro and profiled using methylation and expression arrays. Bioinformatic analysis identified a set of genes both differentially methylated and expressed in scar fibroblasts compared to normal skin fibroblasts .Modulation of expression of two identified genes altered collagen metabolism. This work suggests scar fibroblasts have a distinct methylome and transcriptome that may underpin aberrant collagen production.

KW - DNA methylation

KW - Scarring

KW - Fibrosis

KW - Dermal matrix

KW - Extracellular matrix

KW - Scar skin

KW - methylome

KW - Transcriptome

M3 - Doctoral Thesis

ER -

Investigating the role of epigenetics in scar maintenance

Abstract

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