Investigating the role of epigenetics in scar maintenance

Andrew William Stevenson

    Research output: Thesis › Doctoral Thesis

    Abstract

    Scar is defined by an aberrant dermal matrix containing excess and densely packed collagen .This study aimed to characterise DNA methylation and gene expression patterns that underlie the production of collagen by scar fibroblasts. Dermal fibroblasts from normal skin and scar were cultured in vitro and profiled using methylation and expression arrays. Bioinformatic analysis identified a set of genes both differentially methylated and expressed in scar fibroblasts compared to normal skin fibroblasts .Modulation of expression of two identified genes altered collagen metabolism. This work suggests scar fibroblasts have a distinct methylome and transcriptome that may underpin aberrant collagen production.
    LanguageEnglish
    QualificationDoctor of Philosophy
    Awarding Institution
    • The University of Western Australia
    Award date6 Dec 2016
    Publication statusUnpublished - 2016

    Fingerprint

    Epigenomics
    Cicatrix
    Fibroblasts
    Maintenance
    Collagen
    Skin
    DNA Methylation
    Computational Biology
    Transcriptome
    Methylation
    Genes
    Gene Expression

    Cite this

    Stevenson, Andrew William. / Investigating the role of epigenetics in scar maintenance. 2016.
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    title = "Investigating the role of epigenetics in scar maintenance",
    abstract = "Scar is defined by an aberrant dermal matrix containing excess and densely packed collagen .This study aimed to characterise DNA methylation and gene expression patterns that underlie the production of collagen by scar fibroblasts. Dermal fibroblasts from normal skin and scar were cultured in vitro and profiled using methylation and expression arrays. Bioinformatic analysis identified a set of genes both differentially methylated and expressed in scar fibroblasts compared to normal skin fibroblasts .Modulation of expression of two identified genes altered collagen metabolism. This work suggests scar fibroblasts have a distinct methylome and transcriptome that may underpin aberrant collagen production.",
    keywords = "DNA methylation, Scarring, Fibrosis, Dermal matrix, Extracellular matrix, Scar skin, methylome, Transcriptome",
    author = "Stevenson, {Andrew William}",
    year = "2016",
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    school = "The University of Western Australia",

    }

    Stevenson, AW 2016, 'Investigating the role of epigenetics in scar maintenance', Doctor of Philosophy, The University of Western Australia.

    Investigating the role of epigenetics in scar maintenance. / Stevenson, Andrew William.

    2016.

    Research output: Thesis › Doctoral Thesis

    TY - THES

    T1 - Investigating the role of epigenetics in scar maintenance

    AU - Stevenson, Andrew William

    PY - 2016

    Y1 - 2016

    N2 - Scar is defined by an aberrant dermal matrix containing excess and densely packed collagen .This study aimed to characterise DNA methylation and gene expression patterns that underlie the production of collagen by scar fibroblasts. Dermal fibroblasts from normal skin and scar were cultured in vitro and profiled using methylation and expression arrays. Bioinformatic analysis identified a set of genes both differentially methylated and expressed in scar fibroblasts compared to normal skin fibroblasts .Modulation of expression of two identified genes altered collagen metabolism. This work suggests scar fibroblasts have a distinct methylome and transcriptome that may underpin aberrant collagen production.

    AB - Scar is defined by an aberrant dermal matrix containing excess and densely packed collagen .This study aimed to characterise DNA methylation and gene expression patterns that underlie the production of collagen by scar fibroblasts. Dermal fibroblasts from normal skin and scar were cultured in vitro and profiled using methylation and expression arrays. Bioinformatic analysis identified a set of genes both differentially methylated and expressed in scar fibroblasts compared to normal skin fibroblasts .Modulation of expression of two identified genes altered collagen metabolism. This work suggests scar fibroblasts have a distinct methylome and transcriptome that may underpin aberrant collagen production.

    KW - DNA methylation

    KW - Scarring

    KW - Fibrosis

    KW - Dermal matrix

    KW - Extracellular matrix

    KW - Scar skin

    KW - methylome

    KW - Transcriptome

    M3 - Doctoral Thesis

    ER -

    Stevenson AW. Investigating the role of epigenetics in scar maintenance. 2016.

    Access to Document

    • THESIS - DOCTOR OF PHILOSOPHY - STEVENSON Andrew William - 2016

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