Scar is defined by an aberrant dermal matrix containing excess and densely packed collagen .This study aimed to characterise DNA methylation and gene expression patterns that underlie the production of collagen by scar fibroblasts. Dermal fibroblasts from normal skin and scar were cultured in vitro and profiled using methylation and expression arrays. Bioinformatic analysis identified a set of genes both differentially methylated and expressed in scar fibroblasts compared to normal skin fibroblasts .Modulation of expression of two identified genes altered collagen metabolism. This work suggests scar fibroblasts have a distinct methylome and transcriptome that may underpin aberrant collagen production.
|Qualification||Doctor of Philosophy|
|Award date||6 Dec 2016|
|Publication status||Unpublished - 2016|