Investigating the Molecular Mechanisms of Anaphylaxis

Francesca McGrath

doi:10.26182/1xy0-8z50

Investigating the Molecular Mechanisms of Anaphylaxis

Francesca McGrath

Research output: Thesis › Doctoral Thesis

148 Downloads (Pure)

Abstract

This thesis presents evidence which expands our understanding of anaphylaxis beyond traditional mast cell-histamine pathways. Our approach entailed broad investigation of genes and pathways influencing anaphylaxis, followed by targeted examinations of genes and cells hypothesised to be significant contributors to anaphylaxis. Through this approach, we demonstrated, for the first time, the dysregulation of small nucleolar RNAs, natural killer (NK) cell-related genes, and platelet-related genes during acute anaphylaxis. This elucidates novel pathways potentially driving anaphylaxis, and identifies promising biomarker candidates. Through further interrogation of these genes and associated proteins, we identified novel features of NK cells unique to anaphylaxis-prone individuals.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Bosio, Erika, Supervisor Fatovich, Daniel, Supervisor Woo, Andrew, Supervisor
Thesis sponsors	Australian Government Research Training Program
Award date	27 Nov 2023
DOIs	https://doi.org/10.26182/1xy0-8z50
Publication status	Unpublished - 2023

Access to Document

10.26182/1xy0-8z50

THESIS - DOCTOR OF PHILOSOPHY - McGRATH Francesca Marina - 2023
This work is protected by Copyright. You may print or download ONE copy of this document for the purpose of your own non-commercial research or study. Any other use requires permission from the copyright owner. The Copyright Act requires you to attribute any copyright works you quote or paraphrase.

2 Article

Genes involved in platelet aggregation and activation are downregulated during acute anaphylaxis in humans
McGrath, F. M., Francis, A., Fatovich, D. M., Macdonald, S. P. J., Arendts, G., Woo, A. J. & Bosio, E., 2022, In: Clinical and Translational Immunology. 11, 12, e1435.
Research output: Contribution to journal › Article › peer-review

Open Access
7 Citations (Scopus)
Small nucleolar RNA networks are upregulated during human anaphylaxis
McGrath, F., Francis, A., Fatovich, D., Macdonald, S., Arendts, G., Bosco, A., Woo, A. J. & Bosio, E., Oct 2021, In: Clinical & Experimental Allergy. 51, 10, p. 1310 - 1321 12 p.
Research output: Contribution to journal › Article › peer-review

Open Access
File
5 Citations (Scopus)

191 Downloads (Pure)

Cite this

@phdthesis{196118c251f04013a38895a67a903146,

title = "Investigating the Molecular Mechanisms of Anaphylaxis",

abstract = "This thesis presents evidence which expands our understanding of anaphylaxis beyond traditional mast cell-histamine pathways. Our approach entailed broad investigation of genes and pathways influencing anaphylaxis, followed by targeted examinations of genes and cells hypothesised to be significant contributors to anaphylaxis. Through this approach, we demonstrated, for the first time, the dysregulation of small nucleolar RNAs, natural killer (NK) cell-related genes, and platelet-related genes during acute anaphylaxis. This elucidates novel pathways potentially driving anaphylaxis, and identifies promising biomarker candidates. Through further interrogation of these genes and associated proteins, we identified novel features of NK cells unique to anaphylaxis-prone individuals.",

keywords = "Anaphylaxis, allergy, emergency medicine, immunology, bioinformatics, molecular biology, genomics",

author = "Francesca McGrath",

year = "2023",

doi = "10.26182/1xy0-8z50",

language = "English",

school = "The University of Western Australia",

}

TY - BOOK

T1 - Investigating the Molecular Mechanisms of Anaphylaxis

AU - McGrath, Francesca

PY - 2023

Y1 - 2023

N2 - This thesis presents evidence which expands our understanding of anaphylaxis beyond traditional mast cell-histamine pathways. Our approach entailed broad investigation of genes and pathways influencing anaphylaxis, followed by targeted examinations of genes and cells hypothesised to be significant contributors to anaphylaxis. Through this approach, we demonstrated, for the first time, the dysregulation of small nucleolar RNAs, natural killer (NK) cell-related genes, and platelet-related genes during acute anaphylaxis. This elucidates novel pathways potentially driving anaphylaxis, and identifies promising biomarker candidates. Through further interrogation of these genes and associated proteins, we identified novel features of NK cells unique to anaphylaxis-prone individuals.

AB - This thesis presents evidence which expands our understanding of anaphylaxis beyond traditional mast cell-histamine pathways. Our approach entailed broad investigation of genes and pathways influencing anaphylaxis, followed by targeted examinations of genes and cells hypothesised to be significant contributors to anaphylaxis. Through this approach, we demonstrated, for the first time, the dysregulation of small nucleolar RNAs, natural killer (NK) cell-related genes, and platelet-related genes during acute anaphylaxis. This elucidates novel pathways potentially driving anaphylaxis, and identifies promising biomarker candidates. Through further interrogation of these genes and associated proteins, we identified novel features of NK cells unique to anaphylaxis-prone individuals.

KW - Anaphylaxis

KW - allergy

KW - emergency medicine

KW - immunology

KW - bioinformatics

KW - molecular biology

KW - genomics

U2 - 10.26182/1xy0-8z50

DO - 10.26182/1xy0-8z50

M3 - Doctoral Thesis

ER -

Investigating the Molecular Mechanisms of Anaphylaxis

Abstract

Access to Document

Fingerprint

Research output

Genes involved in platelet aggregation and activation are downregulated during acute anaphylaxis in humans

Small nucleolar RNA networks are upregulated during human anaphylaxis

Cite this