Investigating cooperative activity of PPR proteins in native and synthetic systems

Rose McDowell

Research output: ThesisDoctoral Thesis

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Abstract

Synthetic pentatricopeptide repeat (PPR) proteins make compelling candidates as biological tools to target and bind RNA transcripts. One method to maximise the utility of such tools is through harnessing cooperative activity of PPR proteins. Findings from this study expand knowledge of the evolution of the PPR gene family in early-branching land plants, and highlight the most prolific example of PPR-cooperativity to date, necessary to achieve extensive RNA editing in a hyper-editing species. Additionally, this study provides the first example of a successful PPR-mediated split-effector system in living cells, and an important proof of concept for development of new PPR-based tools.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • The University of Western Australia
Supervisors/Advisors
  • Small, Ian, Supervisor
  • Bond, Charlie, Supervisor
  • Bernath-Levin, Kalia, Supervisor
Thesis sponsors
Award date10 Jun 2022
DOIs
Publication statusUnpublished - 2021

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