Investigating cooperative activity of PPR proteins in native and synthetic systems

Rose McDowell

doi:10.26182/07j0-bh31

Investigating cooperative activity of PPR proteins in native and synthetic systems

Rose McDowell

School of Molecular Sciences

Research output: Thesis › Doctoral Thesis

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Abstract

Synthetic pentatricopeptide repeat (PPR) proteins make compelling candidates as biological tools to target and bind RNA transcripts. One method to maximise the utility of such tools is through harnessing cooperative activity of PPR proteins. Findings from this study expand knowledge of the evolution of the PPR gene family in early-branching land plants, and highlight the most prolific example of PPR-cooperativity to date, necessary to achieve extensive RNA editing in a hyper-editing species. Additionally, this study provides the first example of a successful PPR-mediated split-effector system in living cells, and an important proof of concept for development of new PPR-based tools.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Small, Ian, Supervisor Bond, Charlie, Supervisor Bernath-Levin, Kalia, Supervisor
Thesis sponsors	Australian Government Research Training Program
Award date	10 Jun 2022
DOIs	https://doi.org/10.26182/07j0-bh31
Publication status	Unpublished - 2021

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10.26182/07j0-bh31

THESIS - DOCTOR OF PHILOSOPHY - MCDOWELL, ROSE ISABELLA - 2022
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Cite this

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title = "Investigating cooperative activity of PPR proteins in native and synthetic systems",

abstract = "Synthetic pentatricopeptide repeat (PPR) proteins make compelling candidates as biological tools to target and bind RNA transcripts. One method to maximise the utility of such tools is through harnessing cooperative activity of PPR proteins. Findings from this study expand knowledge of the evolution of the PPR gene family in early-branching land plants, and highlight the most prolific example of PPR-cooperativity to date, necessary to achieve extensive RNA editing in a hyper-editing species. Additionally, this study provides the first example of a successful PPR-mediated split-effector system in living cells, and an important proof of concept for development of new PPR-based tools.",

keywords = "Pentatricopeptide repeat proteins, RNA editing, Gene family evolution, Synthetic RNA-binding proteins",

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AB - Synthetic pentatricopeptide repeat (PPR) proteins make compelling candidates as biological tools to target and bind RNA transcripts. One method to maximise the utility of such tools is through harnessing cooperative activity of PPR proteins. Findings from this study expand knowledge of the evolution of the PPR gene family in early-branching land plants, and highlight the most prolific example of PPR-cooperativity to date, necessary to achieve extensive RNA editing in a hyper-editing species. Additionally, this study provides the first example of a successful PPR-mediated split-effector system in living cells, and an important proof of concept for development of new PPR-based tools.

KW - Pentatricopeptide repeat proteins

KW - RNA editing

KW - Gene family evolution

KW - Synthetic RNA-binding proteins

U2 - 10.26182/07j0-bh31

DO - 10.26182/07j0-bh31

M3 - Doctoral Thesis

ER -

Investigating cooperative activity of PPR proteins in native and synthetic systems

Abstract

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