Intranasal administration of RSV antigen-expressing MCMV elicits robust tissue-resident effector and effector memory CD8+ T cells in the lung

K. M. Morabito, T. R. Ruckwardt, A. J. Redwood, S. M. Moin, D. A. Price, B. S. Graham

Research output: Contribution to journalArticlepeer-review

74 Citations (Scopus)

Abstract

Cytomegalovirus vectors are promising delivery vehicles for vaccine strategies that aim to elicit effector CD8+ T cells. To determine how the route of immunization affects CD8+ T-cell responses in the lungs of mice vaccinated with a murine cytomegalovirus vector expressing the respiratory syncytial virus matrix (M) protein, we infected CB6F1 mice via the intranasal or intraperitoneal route and evaluated the M-specific CD8+ T-cell response at early and late time points. We found that intranasal vaccination generated robust and durable tissue-resident effector and effector memory CD8+ T-cell populations that were undetectable after intraperitoneal vaccination. The generation of these antigen-experienced cells by intranasal vaccination resulted in earlier T-cell responses, interferon gamma secretion, and viral clearance after respiratory syncytial virus challenge. Collectively, these findings validate a novel approach to vaccination that emphasizes the route of delivery as a key determinant of immune priming at the site of vulnerability.

Original languageEnglish
Pages (from-to)545-554
Number of pages10
JournalMucosal Immunology
Volume10
Issue number2
DOIs
Publication statusPublished - 1 Mar 2017
Externally publishedYes

Fingerprint

Dive into the research topics of 'Intranasal administration of RSV antigen-expressing MCMV elicits robust tissue-resident effector and effector memory CD8+ T cells in the lung'. Together they form a unique fingerprint.

Cite this