Intracellular distribution and stability of a luminescent rhenium(i) tricarbonyl tetrazolato complex using epifluorescence microscopy in conjunction with X-ray fluorescence imaging

J. L. Wedding, Tiffany Harris-Brown, C. A. Bader, S. E. Plush, R. Mak, M. Massi, D. A. Brooks, B. Lai, S. Vogt, M. V. Werrett, P. V. Simpson, B. W. Skelton, S. Stagni

Research output: Contribution to journalArticle

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Abstract

Optical epifluorescence microscopy was used in conjunction with X-ray fluorescence imaging to monitor the stability and intracellular distribution of the luminescent rhenium(i) complex fac-[Re(CO)3(phen)L], where phen = 1,10-phenathroline and L = 5-(4-iodophenyl)tetrazolato, in 22Rv1 cells. The rhenium complex showed no signs of ancillary ligand dissociation, a conclusion based on data obtained via X-ray fluorescence imaging aligning iodine and rhenium distributions. A diffuse reticular localisation was detected for the complex in the nuclear/perinuclear region of cells, by either optical or X-ray fluorescence imaging techniques. X-ray fluorescence also showed that the rhenium complex disrupted the homeostasis of some biologically relevant elements, such as chlorine, potassium and zinc.

Original languageEnglish
Pages (from-to)382-390
Number of pages9
JournalMetallomics
Volume9
Issue number4
DOIs
Publication statusPublished - 1 Apr 2017

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Rhenium
Optical Imaging
Microscopy
Microscopic examination
Fluorescence
X-Rays
Imaging techniques
X rays
Chlorine
Carbon Monoxide
Iodine
Optical microscopy
Potassium
Zinc
Homeostasis
Ligands

Cite this

Wedding, J. L. ; Harris-Brown, Tiffany ; Bader, C. A. ; Plush, S. E. ; Mak, R. ; Massi, M. ; Brooks, D. A. ; Lai, B. ; Vogt, S. ; Werrett, M. V. ; Simpson, P. V. ; Skelton, B. W. ; Stagni, S. / Intracellular distribution and stability of a luminescent rhenium(i) tricarbonyl tetrazolato complex using epifluorescence microscopy in conjunction with X-ray fluorescence imaging. In: Metallomics. 2017 ; Vol. 9, No. 4. pp. 382-390.
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abstract = "Optical epifluorescence microscopy was used in conjunction with X-ray fluorescence imaging to monitor the stability and intracellular distribution of the luminescent rhenium(i) complex fac-[Re(CO)3(phen)L], where phen = 1,10-phenathroline and L = 5-(4-iodophenyl)tetrazolato, in 22Rv1 cells. The rhenium complex showed no signs of ancillary ligand dissociation, a conclusion based on data obtained via X-ray fluorescence imaging aligning iodine and rhenium distributions. A diffuse reticular localisation was detected for the complex in the nuclear/perinuclear region of cells, by either optical or X-ray fluorescence imaging techniques. X-ray fluorescence also showed that the rhenium complex disrupted the homeostasis of some biologically relevant elements, such as chlorine, potassium and zinc.",
author = "Wedding, {J. L.} and Tiffany Harris-Brown and Bader, {C. A.} and Plush, {S. E.} and R. Mak and M. Massi and Brooks, {D. A.} and B. Lai and S. Vogt and Werrett, {M. V.} and Simpson, {P. V.} and Skelton, {B. W.} and S. Stagni",
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Wedding, JL, Harris-Brown, T, Bader, CA, Plush, SE, Mak, R, Massi, M, Brooks, DA, Lai, B, Vogt, S, Werrett, MV, Simpson, PV, Skelton, BW & Stagni, S 2017, 'Intracellular distribution and stability of a luminescent rhenium(i) tricarbonyl tetrazolato complex using epifluorescence microscopy in conjunction with X-ray fluorescence imaging' Metallomics, vol. 9, no. 4, pp. 382-390. https://doi.org/10.1039/c6mt00243a

Intracellular distribution and stability of a luminescent rhenium(i) tricarbonyl tetrazolato complex using epifluorescence microscopy in conjunction with X-ray fluorescence imaging. / Wedding, J. L.; Harris-Brown, Tiffany; Bader, C. A.; Plush, S. E.; Mak, R.; Massi, M.; Brooks, D. A.; Lai, B.; Vogt, S.; Werrett, M. V.; Simpson, P. V.; Skelton, B. W.; Stagni, S.

In: Metallomics, Vol. 9, No. 4, 01.04.2017, p. 382-390.

Research output: Contribution to journalArticle

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T1 - Intracellular distribution and stability of a luminescent rhenium(i) tricarbonyl tetrazolato complex using epifluorescence microscopy in conjunction with X-ray fluorescence imaging

AU - Wedding, J. L.

AU - Harris-Brown, Tiffany

AU - Bader, C. A.

AU - Plush, S. E.

AU - Mak, R.

AU - Massi, M.

AU - Brooks, D. A.

AU - Lai, B.

AU - Vogt, S.

AU - Werrett, M. V.

AU - Simpson, P. V.

AU - Skelton, B. W.

AU - Stagni, S.

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AB - Optical epifluorescence microscopy was used in conjunction with X-ray fluorescence imaging to monitor the stability and intracellular distribution of the luminescent rhenium(i) complex fac-[Re(CO)3(phen)L], where phen = 1,10-phenathroline and L = 5-(4-iodophenyl)tetrazolato, in 22Rv1 cells. The rhenium complex showed no signs of ancillary ligand dissociation, a conclusion based on data obtained via X-ray fluorescence imaging aligning iodine and rhenium distributions. A diffuse reticular localisation was detected for the complex in the nuclear/perinuclear region of cells, by either optical or X-ray fluorescence imaging techniques. X-ray fluorescence also showed that the rhenium complex disrupted the homeostasis of some biologically relevant elements, such as chlorine, potassium and zinc.

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