Intra-amniotic endotoxin induces lung maturation by direct effects on the developing respiratory tract in preterm sheep.

T.J. Moss, Ilias Nitsos, B.W. Kramer, M. Ikegami, John Newnham, A.H. Jobe

    Research output: Contribution to journalArticle

    78 Citations (Scopus)

    Abstract

    OBJECTIVE: Our purpose was to determine whether improved preterm lung function caused by intra-amniotic endotoxin treatment requires endotoxin entry into the respiratory tract.STUDY DESIGN: We assessed lung inflammation 2 days after intra-amniotic endotoxin (10 mg, Escherichia coli 055:B5) or saline solution in preterm lambs (123 days' gestation) that had undergone surgery to isolate the gastrointestinal or respiratory systems from the amniotic sac. In other sheep longer-term effects were assessed 7 days after we isolated the fetal respiratory tract and gave endotoxin or saline solution directly to the lungs or into the amniotic sac. We measured pulmonary inflammation, lung function, and surfactant 1 week after treatment (similar to125 days).RESULTS: Pulmonary inflammation was present after intra-amniotic endotoxin only if there was communication between the respiratory tract and the amniotic sac. Lung function was improved and surfactant was increased only in preterm lambs that received direct pulmonary endotoxin.CONCLUSION: Endotoxin causes functional improvement of the preterm lung by direct effects on the developing respiratory system.
    Original languageEnglish
    Pages (from-to)1059-1065
    JournalAmerican Journal of Obstetrics and Gynecology
    Volume187
    Issue number4
    DOIs
    Publication statusPublished - 2002

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