Integrated physical map of bread wheat chromosome arm 7DS to facilitate gene cloning and comparative studies

Zuzana Tulpová, Ming Cheng Luo, Helena Toegelová, Paul Visendi, Satomi Hayashi, Petr Vojta, Etienne Paux, Andrzej Kilian, Michaël Abrouk, Jan Bartoš, Marián Hajdúch, Jacqueline Batley, David Edwards, Jaroslav Doležel, Hana Šimková

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Bread wheat (Triticum aestivum L.) is a staple food for a significant part of the world's population. The growing demand on its production can be satisfied by improving yield and resistance to biotic and abiotic stress. Knowledge of the genome sequence would aid in discovering genes and QTLs underlying these traits and provide a basis for genomics-assisted breeding. Physical maps and BAC clones associated with them have been valuable resources from which to generate a reference genome of bread wheat and to assist map-based gene cloning. As a part of a joint effort coordinated by the International Wheat Genome Sequencing Consortium, we have constructed a BAC-based physical map of bread wheat chromosome arm 7DS consisting of 895 contigs and covering 94% of its estimated length. By anchoring BAC contigs to one radiation hybrid map and three high resolution genetic maps, we assigned 73% of the assembly to a distinct genomic position. This map integration, interconnecting a total of 1713 markers with ordered and sequenced BAC clones from a minimal tiling path, provides a tool to speed up gene cloning in wheat. The process of physical map assembly included the integration of the 7DS physical map with a whole-genome physical map of Aegilops tauschii and a 7DS Bionano genome map, which together enabled efficient scaffolding of physical-map contigs, even in the non-recombining region of the genetic centromere. Moreover, this approach facilitated a comparison of bread wheat and its ancestor at BAC-contig level and revealed a reconstructed region in the 7DS pericentromere.

Original languageEnglish
Pages (from-to)12-19
JournalNew Biotechnology
Volume48
DOIs
Publication statusPublished - 25 Jan 2019

Fingerprint

Cloning
Bread
Chromosomes
Triticum
Organism Cloning
Genes
Genome
Clone Cells
Physical Phenomena
Radiation Hybrid Mapping
Centromere
Genomics
Breeding
Food
Population
Radiation

Cite this

Tulpová, Z., Luo, M. C., Toegelová, H., Visendi, P., Hayashi, S., Vojta, P., ... Šimková, H. (2019). Integrated physical map of bread wheat chromosome arm 7DS to facilitate gene cloning and comparative studies. New Biotechnology, 48, 12-19. https://doi.org/10.1016/j.nbt.2018.03.003
Tulpová, Zuzana ; Luo, Ming Cheng ; Toegelová, Helena ; Visendi, Paul ; Hayashi, Satomi ; Vojta, Petr ; Paux, Etienne ; Kilian, Andrzej ; Abrouk, Michaël ; Bartoš, Jan ; Hajdúch, Marián ; Batley, Jacqueline ; Edwards, David ; Doležel, Jaroslav ; Šimková, Hana. / Integrated physical map of bread wheat chromosome arm 7DS to facilitate gene cloning and comparative studies. In: New Biotechnology. 2019 ; Vol. 48. pp. 12-19.
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Tulpová, Z, Luo, MC, Toegelová, H, Visendi, P, Hayashi, S, Vojta, P, Paux, E, Kilian, A, Abrouk, M, Bartoš, J, Hajdúch, M, Batley, J, Edwards, D, Doležel, J & Šimková, H 2019, 'Integrated physical map of bread wheat chromosome arm 7DS to facilitate gene cloning and comparative studies' New Biotechnology, vol. 48, pp. 12-19. https://doi.org/10.1016/j.nbt.2018.03.003

Integrated physical map of bread wheat chromosome arm 7DS to facilitate gene cloning and comparative studies. / Tulpová, Zuzana; Luo, Ming Cheng; Toegelová, Helena; Visendi, Paul; Hayashi, Satomi; Vojta, Petr; Paux, Etienne; Kilian, Andrzej; Abrouk, Michaël; Bartoš, Jan; Hajdúch, Marián; Batley, Jacqueline; Edwards, David; Doležel, Jaroslav; Šimková, Hana.

In: New Biotechnology, Vol. 48, 25.01.2019, p. 12-19.

Research output: Contribution to journalArticle

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AU - Tulpová, Zuzana

AU - Luo, Ming Cheng

AU - Toegelová, Helena

AU - Visendi, Paul

AU - Hayashi, Satomi

AU - Vojta, Petr

AU - Paux, Etienne

AU - Kilian, Andrzej

AU - Abrouk, Michaël

AU - Bartoš, Jan

AU - Hajdúch, Marián

AU - Batley, Jacqueline

AU - Edwards, David

AU - Doležel, Jaroslav

AU - Šimková, Hana

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