Integrated multi-channel optical system for bacteria characterization and its potential use for monitoring of environmental bacteria

Igor Buzalewicz, Agnieszka Suchwalko, Pawel Trzcinski, Lidia Sas-Paszt, Beata Sumorok, Katarzyna Kowal, Ryszard Kozera, Alina Wieliczko, Halina Podbielska

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The potential use of a novel multichannel optical system towards fast and nondestructive bacteria identification and its application for environmental bacteria characterisation on the strain level is presented. It is the first attempt to use the proposed optical method to study various bacteria species (Gram-negative, Gram-positive) commonly present in the environment. The novel configuration of the optical system enables multichannel examination of bacterial colonies and provides additional functionality such as registration of two-dimensional (2D) distribution of monochromatic transmission coefficient of examined colonies, what can be used as a novel optical signature for bacteria characterization. Performed statistical analysis indicates that it is possible to identify representatives of environmental soil bacteria on the species level with the 98.51% accuracy and in case of two strains of Rahnella aquatilis bacteria on the strain level with the 98.8% accuracy. The proposed method is an alternative to the currently used preliminary bacteria examination in environment safety control with the advantage of being fast, reliable, nondestructive and requiring minimal sample preparation. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

Original languageEnglish
Pages (from-to)1165-1183
Number of pages19
JournalBiomedical Optics Express
Issue number3
Publication statusPublished - 1 Mar 2019

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