Insulin antagonises pigment epithelium-derived factor (PEDF)-induced modulation of lineage commitment of myocytes and heterotrophic ossification

Revathy Carnagarin, Mina Elahy, Arun M Dharmarajan, Crispin R Dass

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


Extensive bone defects arising as a result of trauma, infection and tumour resection and other bone pathologies necessitates the identification of effective strategies in the form of tissue engineering, gene therapy and osteoinductive agents to enhance the bone repair process. PEDF is a multifunctional glycoprotein which plays an important role in regulating osteoblastic differentiation and bone formation. PEDF treatment of mice and human skeletal myocytes at physiological concentration inhibited myogenic differentiation and activated Erk1/2 MAPK- dependent osteogenic transdifferentiation of myocytes. In mice, insulin, a promoter of bone regeneration, attenuated PEDF-induced expression of osteogenic markers such as osteocalcin, alkaline phosphatase and mineralisation for bone formation in the muscle and surrounding adipose tissue. These results provide new insights into the molecular aspects of the antagonising effect of insulin on PEDF-dependent modulation of the differentiation commitment of musculoskeletal environment into osteogenesis, and suggest that PEDF may be developed as an effective clinical therapy for bone regeneration as its heterotopic ossification can be controlled via co-administration of insulin.

Original languageEnglish
Pages (from-to)159-166
Number of pages8
JournalMolecular and Cellular Endocrinology
Publication statusPublished - 5 Sept 2018


Dive into the research topics of 'Insulin antagonises pigment epithelium-derived factor (PEDF)-induced modulation of lineage commitment of myocytes and heterotrophic ossification'. Together they form a unique fingerprint.
  • Role of histone acetyltransferase inhibitors in cancer therapy

    Shanmugam, M. K., Dharmarajan, A., Warrier, S., Bishayee, A., Kumar, A. P., Sethi, G. & Ahn, K. S., Jan 2021, Apoptosis in Health and Disease - Part A. UK: Academic Press, Vol. 125. p. 149-191 43 p. (Advances in Protein Chemistry and Structural Biology; vol. 125).

    Research output: Chapter in Book/Conference paperChapterpeer-review

    13 Citations (Scopus)

Cite this