Installation of C4 photosynthetic pathway enzymes in rice using a single construct

Maria Ermakova, Stéphanie Arrivault, Rita Giuliani, Florence Danila, Hugo Alonso-Cantabrana, Daniela Vlad, Hirofumi Ishihara, Regina Feil, Manuela Guenther, Gian Luca Borghi, Sarah Covshoff, Martha Ludwig, Asaph B. Cousins, Jane A. Langdale, Steven Kelly, John E. Lunn, Mark Stitt, Susanne von Caemmerer, Robert T. Furbank

Research output: Contribution to journalArticlepeer-review

69 Citations (Scopus)


Introduction of a C4 photosynthetic mechanism into C3 crops offers an opportunity to improve photosynthetic efficiency, biomass and yield in addition to potentially improving nitrogen and water use efficiency. To create a two-cell metabolic prototype for an NADP-malic enzyme type C4 rice, we transformed Oryza sativa spp.japonica cultivar Kitaake with a single construct containing the coding regions of carbonic anhydrase, phosphoenolpyruvate (PEP) carboxylase, NADP-malate dehydrogenase, pyruvate orthophosphate dikinase and NADP-malic enzyme from Zea mays, driven by cell-preferential promoters. Gene expression, protein accumulation and enzyme activity were confirmed for all five transgenes, and intercellular localization of proteins was analysed. 13CO2 labelling demonstrated a 10-fold increase in flux though PEP carboxylase, exceeding the increase in measured in vitro enzyme activity, and estimated to be about 2% of the maize photosynthetic flux. Flux from malate via pyruvate to PEP remained low, commensurate with the low NADP-malic enzyme activity observed in the transgenic lines. Physiological perturbations were minor and RNA sequencing revealed no substantive effects of transgene expression on other endogenous rice transcripts associated with photosynthesis. These results provide promise that, with enhanced levels of the C4 proteins introduced thus far, a functional C4 pathway is achievable in rice.

Original languageEnglish
Pages (from-to)575-588
Number of pages14
JournalPlant Biotechnology Journal
Issue number3
Early online date5 Oct 2020
Publication statusPublished - Mar 2021


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