Inhibition of nitric oxide synthesis potentiates apoptosis in the rabbit corpus luteum

S. Preutthipan, S.H. Chen, J.L. Tilly, K. Kugu, Ricky Lareu, Arunasalam Dharmarajan

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13 Citations (Scopus)


To determine if nitric oxide (NO) plays a role in corpus luteum (CL) physiology by affecting progesterone secretion or luteal apoptosis, an in-vitro pseudopregnant rabbit ovarian perfusion system was used to measure the effects of an inhibitor of NO synthesis, N-G-nitro-L-arginine methyl ester (L-NAME), on progesterone secretion and corpus luteal apoptosis as measured by internucleosomal DNA breakdown. Pseudopregnant rabbit ovaries perfused in vitro with L-NAME did not demonstrate any significant differences compared with control ovaries in progesterone secretion. However, apoptosis, as measured by internucleosomal breakdown, was significantly increased in L-NAME-perfused CL compared with controls. While NO does not appear to directly affect progesterone secretion, there does appear to be a role for NO in CL maintenance, or a role for inhibition of NO production in CL regression.
Original languageEnglish
Pages (from-to)264-270
JournalReproductive Biomedicine Online
Issue number3
Publication statusPublished - 2004


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