INHERITANCE AND MECHANISM OF RESISTANCE TO HERBICIDES INHIBITING ACETOLACTATE SYNTHASE IN SONCHUS-OLERACEUS L

P Boutsalis, Stephen Powles

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    38 Citations (Scopus)

    Abstract

    A biotype of Sonchus oleraceus L. (Compositae) has developed resistance to herbicides inhibiting acetolactate synthase (ALS) following field selection with chlorsulfuron for 8 consecutive years. The aim of this study was to determine the inheritance and mechanism of resistance in this biotype. Determination of ALS activity and inhibition kinetics revealed that K-m and V-max did not vary greatly between the resistant and susceptible biotypes. ALS extracted from the resistant biotype was resistant to five ALS-inhibiting herbicides in an in vitro assay. ALS activity from the resistant biotype was 14 19, 2, 3 and 3 times more resistant to inhibition by chlorsulfuron, sulfometuron, imazethapyr, imazapyr and flumetsulam, respectively, than the susceptible biotype. Hybrids between the resistant and a susceptible biotype were produced, and inheritance was followed through the F-1, F-2 and F-3 generations. F-1 hybrids displayed a uniform intermediate level of resistance between resistant and susceptible parents. Three distinct phenotypes, resistant, intermediate and susceptible, were identified in the F-2 generation following chlorsulfuron application. A segregation ratio of 1:2: 1 was observed, indicative of the action of a single, nuclear, incompletely dominant gene. F-3 families, derived from intermediate F-2 individuals, segregated in a similar manner. Resistance to herbicides inhibiting ALS in this biotype of S. oleraceus is due to the effect of a single gene coding for a resistant form of the target enzyme, ALS.
    Original languageEnglish
    Pages (from-to)242-247
    JournalTheoretical and Applied Genetics: international journal of plant breeding research
    Volume91
    Publication statusPublished - 1995

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    Sonchus
    Acetolactate Synthase
    Herbicide Resistance
    Sonchus oleraceus
    acetolactate synthase
    herbicide resistance
    biotypes
    inheritance (genetics)
    chlorsulfuron
    flumetsulam
    Dominant Genes
    sulfometuron
    Asteraceae
    imazapyr
    imazethapyr
    Herbicides
    dominant genes
    resistance mechanisms
    herbicides
    Phenotype

    Cite this

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    title = "INHERITANCE AND MECHANISM OF RESISTANCE TO HERBICIDES INHIBITING ACETOLACTATE SYNTHASE IN SONCHUS-OLERACEUS L",
    abstract = "A biotype of Sonchus oleraceus L. (Compositae) has developed resistance to herbicides inhibiting acetolactate synthase (ALS) following field selection with chlorsulfuron for 8 consecutive years. The aim of this study was to determine the inheritance and mechanism of resistance in this biotype. Determination of ALS activity and inhibition kinetics revealed that K-m and V-max did not vary greatly between the resistant and susceptible biotypes. ALS extracted from the resistant biotype was resistant to five ALS-inhibiting herbicides in an in vitro assay. ALS activity from the resistant biotype was 14 19, 2, 3 and 3 times more resistant to inhibition by chlorsulfuron, sulfometuron, imazethapyr, imazapyr and flumetsulam, respectively, than the susceptible biotype. Hybrids between the resistant and a susceptible biotype were produced, and inheritance was followed through the F-1, F-2 and F-3 generations. F-1 hybrids displayed a uniform intermediate level of resistance between resistant and susceptible parents. Three distinct phenotypes, resistant, intermediate and susceptible, were identified in the F-2 generation following chlorsulfuron application. A segregation ratio of 1:2: 1 was observed, indicative of the action of a single, nuclear, incompletely dominant gene. F-3 families, derived from intermediate F-2 individuals, segregated in a similar manner. Resistance to herbicides inhibiting ALS in this biotype of S. oleraceus is due to the effect of a single gene coding for a resistant form of the target enzyme, ALS.",
    author = "P Boutsalis and Stephen Powles",
    year = "1995",
    language = "English",
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    AU - Powles, Stephen

    PY - 1995

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    N2 - A biotype of Sonchus oleraceus L. (Compositae) has developed resistance to herbicides inhibiting acetolactate synthase (ALS) following field selection with chlorsulfuron for 8 consecutive years. The aim of this study was to determine the inheritance and mechanism of resistance in this biotype. Determination of ALS activity and inhibition kinetics revealed that K-m and V-max did not vary greatly between the resistant and susceptible biotypes. ALS extracted from the resistant biotype was resistant to five ALS-inhibiting herbicides in an in vitro assay. ALS activity from the resistant biotype was 14 19, 2, 3 and 3 times more resistant to inhibition by chlorsulfuron, sulfometuron, imazethapyr, imazapyr and flumetsulam, respectively, than the susceptible biotype. Hybrids between the resistant and a susceptible biotype were produced, and inheritance was followed through the F-1, F-2 and F-3 generations. F-1 hybrids displayed a uniform intermediate level of resistance between resistant and susceptible parents. Three distinct phenotypes, resistant, intermediate and susceptible, were identified in the F-2 generation following chlorsulfuron application. A segregation ratio of 1:2: 1 was observed, indicative of the action of a single, nuclear, incompletely dominant gene. F-3 families, derived from intermediate F-2 individuals, segregated in a similar manner. Resistance to herbicides inhibiting ALS in this biotype of S. oleraceus is due to the effect of a single gene coding for a resistant form of the target enzyme, ALS.

    AB - A biotype of Sonchus oleraceus L. (Compositae) has developed resistance to herbicides inhibiting acetolactate synthase (ALS) following field selection with chlorsulfuron for 8 consecutive years. The aim of this study was to determine the inheritance and mechanism of resistance in this biotype. Determination of ALS activity and inhibition kinetics revealed that K-m and V-max did not vary greatly between the resistant and susceptible biotypes. ALS extracted from the resistant biotype was resistant to five ALS-inhibiting herbicides in an in vitro assay. ALS activity from the resistant biotype was 14 19, 2, 3 and 3 times more resistant to inhibition by chlorsulfuron, sulfometuron, imazethapyr, imazapyr and flumetsulam, respectively, than the susceptible biotype. Hybrids between the resistant and a susceptible biotype were produced, and inheritance was followed through the F-1, F-2 and F-3 generations. F-1 hybrids displayed a uniform intermediate level of resistance between resistant and susceptible parents. Three distinct phenotypes, resistant, intermediate and susceptible, were identified in the F-2 generation following chlorsulfuron application. A segregation ratio of 1:2: 1 was observed, indicative of the action of a single, nuclear, incompletely dominant gene. F-3 families, derived from intermediate F-2 individuals, segregated in a similar manner. Resistance to herbicides inhibiting ALS in this biotype of S. oleraceus is due to the effect of a single gene coding for a resistant form of the target enzyme, ALS.

    M3 - Article

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