Infective respiratory syncytial virus is present in human cord blood samples and most prevalent during winter months

Angela Mary Fonceca, Abha Chopra, Avram Levy, Paul Stanton Noakes, Matthew Wee Peng Poh, Natasha Leanne Bear, Susan Prescott, Mark Lloyd Everard

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background Human respiratory syncytial virus (RSV) remains the most common cause of severe lower respiratory tract disease amongst infants, and continues to cause annual epidemics of respiratory disease every winter worldwide. Demonstrating placental transmission of viable RSV in human samples is a major paradigm shift in respiratory routes considered likely for RSV transmission. Methods Droplet digital PCR (ddPCR) was used to identify RSV present in cord blood mononucleocytes (CBM). CBMs testing positive for RSV were treated with phytohemagglutinin (PHA), PHA and nitric oxide (NO) or PHA, NO and palivizumab, and co-cultured with HeLa cell monolayers. Subsequent immuno-staining for RSV was used to visualize infective viral plaques. Results RSV was detected in 26 of 45 samples (57.7%) by ddPCR. CBM's collected in winter were more likely to test positive for RSV (17/21 samples, risk = 80%, OR = 7.08; 95% CI 1.80± 27.80; p = 0.005) compared to non-winter months (9/24 samples, 37.5%). RSV plaques were observed in non-Treated and treated co-cultured HeLa monolayers. Conclusions Demonstrating active RSV in CBMs suggests in utero transmission of infective virus to the fetus without causing overt disease. This is likely to have an important impact on immune development as well as future virus-host interactions, thereby warranting further investigation.

Original languageEnglish
Article numbere0173738
JournalPLoS One
Volume12
Issue number4
DOIs
Publication statusPublished - 1 Apr 2017

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Respiratory Syncytial Viruses
Fetal Blood
Viruses
Blood
viruses
winter
blood
Phytohemagglutinins
phytohemagglutinin
Human respiratory syncytial virus
sampling
Pulmonary diseases
respiratory tract diseases
Nitric Oxide
droplets
nitric oxide
Respiratory Tract Diseases
Polymerase Chain Reaction
Monolayers
HeLa Cells

Cite this

@article{d49306208f9941d88d55a837ed730e84,
title = "Infective respiratory syncytial virus is present in human cord blood samples and most prevalent during winter months",
abstract = "Background Human respiratory syncytial virus (RSV) remains the most common cause of severe lower respiratory tract disease amongst infants, and continues to cause annual epidemics of respiratory disease every winter worldwide. Demonstrating placental transmission of viable RSV in human samples is a major paradigm shift in respiratory routes considered likely for RSV transmission. Methods Droplet digital PCR (ddPCR) was used to identify RSV present in cord blood mononucleocytes (CBM). CBMs testing positive for RSV were treated with phytohemagglutinin (PHA), PHA and nitric oxide (NO) or PHA, NO and palivizumab, and co-cultured with HeLa cell monolayers. Subsequent immuno-staining for RSV was used to visualize infective viral plaques. Results RSV was detected in 26 of 45 samples (57.7{\%}) by ddPCR. CBM's collected in winter were more likely to test positive for RSV (17/21 samples, risk = 80{\%}, OR = 7.08; 95{\%} CI 1.80± 27.80; p = 0.005) compared to non-winter months (9/24 samples, 37.5{\%}). RSV plaques were observed in non-Treated and treated co-cultured HeLa monolayers. Conclusions Demonstrating active RSV in CBMs suggests in utero transmission of infective virus to the fetus without causing overt disease. This is likely to have an important impact on immune development as well as future virus-host interactions, thereby warranting further investigation.",
author = "Fonceca, {Angela Mary} and Abha Chopra and Avram Levy and Noakes, {Paul Stanton} and Poh, {Matthew Wee Peng} and Bear, {Natasha Leanne} and Susan Prescott and Everard, {Mark Lloyd}",
year = "2017",
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Infective respiratory syncytial virus is present in human cord blood samples and most prevalent during winter months. / Fonceca, Angela Mary; Chopra, Abha; Levy, Avram; Noakes, Paul Stanton; Poh, Matthew Wee Peng; Bear, Natasha Leanne; Prescott, Susan; Everard, Mark Lloyd.

In: PLoS One, Vol. 12, No. 4, e0173738, 01.04.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Infective respiratory syncytial virus is present in human cord blood samples and most prevalent during winter months

AU - Fonceca, Angela Mary

AU - Chopra, Abha

AU - Levy, Avram

AU - Noakes, Paul Stanton

AU - Poh, Matthew Wee Peng

AU - Bear, Natasha Leanne

AU - Prescott, Susan

AU - Everard, Mark Lloyd

PY - 2017/4/1

Y1 - 2017/4/1

N2 - Background Human respiratory syncytial virus (RSV) remains the most common cause of severe lower respiratory tract disease amongst infants, and continues to cause annual epidemics of respiratory disease every winter worldwide. Demonstrating placental transmission of viable RSV in human samples is a major paradigm shift in respiratory routes considered likely for RSV transmission. Methods Droplet digital PCR (ddPCR) was used to identify RSV present in cord blood mononucleocytes (CBM). CBMs testing positive for RSV were treated with phytohemagglutinin (PHA), PHA and nitric oxide (NO) or PHA, NO and palivizumab, and co-cultured with HeLa cell monolayers. Subsequent immuno-staining for RSV was used to visualize infective viral plaques. Results RSV was detected in 26 of 45 samples (57.7%) by ddPCR. CBM's collected in winter were more likely to test positive for RSV (17/21 samples, risk = 80%, OR = 7.08; 95% CI 1.80± 27.80; p = 0.005) compared to non-winter months (9/24 samples, 37.5%). RSV plaques were observed in non-Treated and treated co-cultured HeLa monolayers. Conclusions Demonstrating active RSV in CBMs suggests in utero transmission of infective virus to the fetus without causing overt disease. This is likely to have an important impact on immune development as well as future virus-host interactions, thereby warranting further investigation.

AB - Background Human respiratory syncytial virus (RSV) remains the most common cause of severe lower respiratory tract disease amongst infants, and continues to cause annual epidemics of respiratory disease every winter worldwide. Demonstrating placental transmission of viable RSV in human samples is a major paradigm shift in respiratory routes considered likely for RSV transmission. Methods Droplet digital PCR (ddPCR) was used to identify RSV present in cord blood mononucleocytes (CBM). CBMs testing positive for RSV were treated with phytohemagglutinin (PHA), PHA and nitric oxide (NO) or PHA, NO and palivizumab, and co-cultured with HeLa cell monolayers. Subsequent immuno-staining for RSV was used to visualize infective viral plaques. Results RSV was detected in 26 of 45 samples (57.7%) by ddPCR. CBM's collected in winter were more likely to test positive for RSV (17/21 samples, risk = 80%, OR = 7.08; 95% CI 1.80± 27.80; p = 0.005) compared to non-winter months (9/24 samples, 37.5%). RSV plaques were observed in non-Treated and treated co-cultured HeLa monolayers. Conclusions Demonstrating active RSV in CBMs suggests in utero transmission of infective virus to the fetus without causing overt disease. This is likely to have an important impact on immune development as well as future virus-host interactions, thereby warranting further investigation.

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