INDUKSI KALUS DARI EMBRIO PADI MERAH (ORYZA SATIVA CV BARAK CENANA) MENGGUNAKAN ZAT PENGATUR TUMBUH 2,4 D

Ida Artadana; Popy Hardjo; Gilang Bintang Fajar Suhono

INDUKSI KALUS DARI EMBRIO PADI MERAH (ORYZA SATIVA CV BARAK CENANA) MENGGUNAKAN ZAT PENGATUR TUMBUH 2,4 D

Translated title of the contribution: Callus induction from red rice embryo (Oryza sativa Cv Barak Cenana) using 2,4 D plant growth regulator

Ida Artadana, Popy Hardjo, Gilang Bintang Fajar Suhono

Research output: Chapter in Book/Conference paper › Conference paper

Abstract

The aim of this study was to evaluate the effect of 2,4 D on callus production from the red rice (Oryza sativa cv. Barak Cenana) embryo. Rice seeds were dehusked by hands, sterilized using 70% alcohol, 25% Clorox, and 5% Clorox, respectively. Sterilized-seeds were cultured in MS media with additional 1 ppm, 2 ppm, or 3 ppm 2,4 D. Each bottle contains three seeds and the experiment was done with three replications for every treatment. One hundred percent of embryo produced callus in all of those three types of media. All calluses were emerged 18 days after cultured and located at the base of shoot. They are friable calluses with white yellowish colour. After 35 days of culture, embryo in MS media with addition of 1 ppm 2,4 D produced the highest callus fresh weight. Fresh weight of the callus in MS media with addition of 1 ppm, 2 ppm, and 3
ppm 2,4 D were 241,9 ± 57,9; 171,8 ± 30,9; and 156,7 ± 26,3 mg/bottle, respectively. In the future, MS media with addition 1 ppm 2,4 D can be chosen to induce embryo derived callus from Barak Cenana red rice. Barak Cenana embryo derived callus induced by 2,4 D is potentially regenerated to red rice plant.

Translated title of the contribution	Callus induction from red rice embryo (Oryza sativa Cv Barak Cenana) using 2,4 D plant growth regulator
Original language	Other
Title of host publication	KUMPULAN MAKALAH (PROCEEDING) SEMINAR NASIONAL BIODIVERSITAS VI
Subtitle of host publication	Biodiversitas untuk Pembangunan Berkelanjutan - Keanekaragaman Hayati Indonesia dan Perannya dalam Menunjang Kemandirian Bangsa
Editors	Alfiah Hayati, Dwi Winarni, H. Hery Purnobasuki, Ni Matuzahroh, Thin Soedarti, Eko Prasetyo Kuncoro
Publisher	Universitas Airlangga
Chapter	II
Pages	67 - 73
Number of pages	1233
ISBN (Electronic)	978-979-98109-5-3
Publication status	Published - 3 Sep 2016
Externally published	Yes

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https://biologi.fst.unair.ac.id/wp-content/uploads/2017/06/PROSIDING-SEMINAR-NASIONAL-BIODIVERSITAS-6-_FINAL.pdf

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Artadana, I., Hardjo, P., & Suhono, G. B. F. (2016). INDUKSI KALUS DARI EMBRIO PADI MERAH (ORYZA SATIVA CV BARAK CENANA) MENGGUNAKAN ZAT PENGATUR TUMBUH 2,4 D. In A. Hayati, D. Winarni, H. H. Purnobasuki, N. Matuzahroh, T. Soedarti, & E. P. Kuncoro (Eds.), KUMPULAN MAKALAH (PROCEEDING) SEMINAR NASIONAL BIODIVERSITAS VI: Biodiversitas untuk Pembangunan Berkelanjutan - Keanekaragaman Hayati Indonesia dan Perannya dalam Menunjang Kemandirian Bangsa (pp. 67 - 73). Universitas Airlangga. https://biologi.fst.unair.ac.id/wp-content/uploads/2017/06/PROSIDING-SEMINAR-NASIONAL-BIODIVERSITAS-6-_FINAL.pdf

Artadana, Ida ; Hardjo, Popy ; Suhono, Gilang Bintang Fajar. / INDUKSI KALUS DARI EMBRIO PADI MERAH (ORYZA SATIVA CV BARAK CENANA) MENGGUNAKAN ZAT PENGATUR TUMBUH 2,4 D. KUMPULAN MAKALAH (PROCEEDING) SEMINAR NASIONAL BIODIVERSITAS VI: Biodiversitas untuk Pembangunan Berkelanjutan - Keanekaragaman Hayati Indonesia dan Perannya dalam Menunjang Kemandirian Bangsa. editor / Alfiah Hayati ; Dwi Winarni ; H. Hery Purnobasuki ; Ni Matuzahroh ; Thin Soedarti ; Eko Prasetyo Kuncoro. Universitas Airlangga, 2016. pp. 67 - 73

@inproceedings{8c3b8d5486d844d58916506dd4cc20b2,

title = "INDUKSI KALUS DARI EMBRIO PADI MERAH (ORYZA SATIVA CV BARAK CENANA) MENGGUNAKAN ZAT PENGATUR TUMBUH 2,4 D",

abstract = "The aim of this study was to evaluate the effect of 2,4 D on callus production from the red rice (Oryza sativa cv. Barak Cenana) embryo. Rice seeds were dehusked by hands, sterilized using 70% alcohol, 25% Clorox, and 5% Clorox, respectively. Sterilized-seeds were cultured in MS media with additional 1 ppm, 2 ppm, or 3 ppm 2,4 D. Each bottle contains three seeds and the experiment was done with three replications for every treatment. One hundred percent of embryo produced callus in all of those three types of media. All calluses were emerged 18 days after cultured and located at the base of shoot. They are friable calluses with white yellowish colour. After 35 days of culture, embryo in MS media with addition of 1 ppm 2,4 D produced the highest callus fresh weight. Fresh weight of the callus in MS media with addition of 1 ppm, 2 ppm, and 3ppm 2,4 D were 241,9 ± 57,9; 171,8 ± 30,9; and 156,7 ± 26,3 mg/bottle, respectively. In the future, MS media with addition 1 ppm 2,4 D can be chosen to induce embryo derived callus from Barak Cenana red rice. Barak Cenana embryo derived callus induced by 2,4 D is potentially regenerated to red rice plant.",

author = "Ida Artadana and Popy Hardjo and Suhono, {Gilang Bintang Fajar}",

year = "2016",

month = sep,

day = "3",

language = "Other",

pages = "67 -- 73",

editor = "Hayati, {Alfiah } and Winarni, {Dwi } and Purnobasuki, { H. Hery } and Ni Matuzahroh and Soedarti, {Thin } and Kuncoro, {Eko Prasetyo }",

booktitle = "KUMPULAN MAKALAH (PROCEEDING) SEMINAR NASIONAL BIODIVERSITAS VI",

publisher = "Universitas Airlangga",

}

Artadana, I, Hardjo, P & Suhono, GBF 2016, INDUKSI KALUS DARI EMBRIO PADI MERAH (ORYZA SATIVA CV BARAK CENANA) MENGGUNAKAN ZAT PENGATUR TUMBUH 2,4 D. in A Hayati, D Winarni, HH Purnobasuki, N Matuzahroh, T Soedarti & EP Kuncoro (eds), KUMPULAN MAKALAH (PROCEEDING) SEMINAR NASIONAL BIODIVERSITAS VI: Biodiversitas untuk Pembangunan Berkelanjutan - Keanekaragaman Hayati Indonesia dan Perannya dalam Menunjang Kemandirian Bangsa. Universitas Airlangga, pp. 67 - 73. <https://biologi.fst.unair.ac.id/wp-content/uploads/2017/06/PROSIDING-SEMINAR-NASIONAL-BIODIVERSITAS-6-_FINAL.pdf>

INDUKSI KALUS DARI EMBRIO PADI MERAH (ORYZA SATIVA CV BARAK CENANA) MENGGUNAKAN ZAT PENGATUR TUMBUH 2,4 D. / Artadana, Ida; Hardjo, Popy; Suhono, Gilang Bintang Fajar.

KUMPULAN MAKALAH (PROCEEDING) SEMINAR NASIONAL BIODIVERSITAS VI: Biodiversitas untuk Pembangunan Berkelanjutan - Keanekaragaman Hayati Indonesia dan Perannya dalam Menunjang Kemandirian Bangsa. ed. / Alfiah Hayati; Dwi Winarni; H. Hery Purnobasuki; Ni Matuzahroh; Thin Soedarti; Eko Prasetyo Kuncoro. Universitas Airlangga, 2016. p. 67 - 73.

Research output: Chapter in Book/Conference paper › Conference paper

TY - GEN

T1 - INDUKSI KALUS DARI EMBRIO PADI MERAH (ORYZA SATIVA CV BARAK CENANA) MENGGUNAKAN ZAT PENGATUR TUMBUH 2,4 D

AU - Artadana, Ida

AU - Hardjo, Popy

AU - Suhono, Gilang Bintang Fajar

PY - 2016/9/3

Y1 - 2016/9/3

N2 - The aim of this study was to evaluate the effect of 2,4 D on callus production from the red rice (Oryza sativa cv. Barak Cenana) embryo. Rice seeds were dehusked by hands, sterilized using 70% alcohol, 25% Clorox, and 5% Clorox, respectively. Sterilized-seeds were cultured in MS media with additional 1 ppm, 2 ppm, or 3 ppm 2,4 D. Each bottle contains three seeds and the experiment was done with three replications for every treatment. One hundred percent of embryo produced callus in all of those three types of media. All calluses were emerged 18 days after cultured and located at the base of shoot. They are friable calluses with white yellowish colour. After 35 days of culture, embryo in MS media with addition of 1 ppm 2,4 D produced the highest callus fresh weight. Fresh weight of the callus in MS media with addition of 1 ppm, 2 ppm, and 3ppm 2,4 D were 241,9 ± 57,9; 171,8 ± 30,9; and 156,7 ± 26,3 mg/bottle, respectively. In the future, MS media with addition 1 ppm 2,4 D can be chosen to induce embryo derived callus from Barak Cenana red rice. Barak Cenana embryo derived callus induced by 2,4 D is potentially regenerated to red rice plant.

AB - The aim of this study was to evaluate the effect of 2,4 D on callus production from the red rice (Oryza sativa cv. Barak Cenana) embryo. Rice seeds were dehusked by hands, sterilized using 70% alcohol, 25% Clorox, and 5% Clorox, respectively. Sterilized-seeds were cultured in MS media with additional 1 ppm, 2 ppm, or 3 ppm 2,4 D. Each bottle contains three seeds and the experiment was done with three replications for every treatment. One hundred percent of embryo produced callus in all of those three types of media. All calluses were emerged 18 days after cultured and located at the base of shoot. They are friable calluses with white yellowish colour. After 35 days of culture, embryo in MS media with addition of 1 ppm 2,4 D produced the highest callus fresh weight. Fresh weight of the callus in MS media with addition of 1 ppm, 2 ppm, and 3ppm 2,4 D were 241,9 ± 57,9; 171,8 ± 30,9; and 156,7 ± 26,3 mg/bottle, respectively. In the future, MS media with addition 1 ppm 2,4 D can be chosen to induce embryo derived callus from Barak Cenana red rice. Barak Cenana embryo derived callus induced by 2,4 D is potentially regenerated to red rice plant.

M3 - Conference paper

SP - 67

EP - 73

BT - KUMPULAN MAKALAH (PROCEEDING) SEMINAR NASIONAL BIODIVERSITAS VI

A2 - Hayati, Alfiah

A2 - Winarni, Dwi

A2 - Purnobasuki, H. Hery

A2 - Matuzahroh, Ni

A2 - Soedarti, Thin

A2 - Kuncoro, Eko Prasetyo

PB - Universitas Airlangga

ER -

Artadana I, Hardjo P, Suhono GBF. INDUKSI KALUS DARI EMBRIO PADI MERAH (ORYZA SATIVA CV BARAK CENANA) MENGGUNAKAN ZAT PENGATUR TUMBUH 2,4 D. In Hayati A, Winarni D, Purnobasuki HH, Matuzahroh N, Soedarti T, Kuncoro EP, editors, KUMPULAN MAKALAH (PROCEEDING) SEMINAR NASIONAL BIODIVERSITAS VI: Biodiversitas untuk Pembangunan Berkelanjutan - Keanekaragaman Hayati Indonesia dan Perannya dalam Menunjang Kemandirian Bangsa. Universitas Airlangga. 2016. p. 67 - 73

INDUKSI KALUS DARI EMBRIO PADI MERAH (ORYZA SATIVA CV BARAK CENANA) MENGGUNAKAN ZAT PENGATUR TUMBUH 2,4 D

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