INDUKSI KALUS DARI EMBRIO PADI MERAH (ORYZA SATIVA CV BARAK CENANA) MENGGUNAKAN ZAT PENGATUR TUMBUH 2,4 D

Translated title of the contribution: Callus induction from red rice embryo (Oryza sativa Cv Barak Cenana) using 2,4 D plant growth regulator

Ida Artadana, Popy Hardjo, Gilang Bintang Fajar Suhono

Research output: Chapter in Book/Conference paperConference paper

Abstract

The aim of this study was to evaluate the effect of 2,4 D on callus production from the red rice (Oryza sativa cv. Barak Cenana) embryo. Rice seeds were dehusked by hands, sterilized using 70% alcohol, 25% Clorox, and 5% Clorox, respectively. Sterilized-seeds were cultured in MS media with additional 1 ppm, 2 ppm, or 3 ppm 2,4 D. Each bottle contains three seeds and the experiment was done with three replications for every treatment. One hundred percent of embryo produced callus in all of those three types of media. All calluses were emerged 18 days after cultured and located at the base of shoot. They are friable calluses with white yellowish colour. After 35 days of culture, embryo in MS media with addition of 1 ppm 2,4 D produced the highest callus fresh weight. Fresh weight of the callus in MS media with addition of 1 ppm, 2 ppm, and 3
ppm 2,4 D were 241,9 ± 57,9; 171,8 ± 30,9; and 156,7 ± 26,3 mg/bottle, respectively. In the future, MS media with addition 1 ppm 2,4 D can be chosen to induce embryo derived callus from Barak Cenana red rice. Barak Cenana embryo derived callus induced by 2,4 D is potentially regenerated to red rice plant.
Translated title of the contributionCallus induction from red rice embryo (Oryza sativa Cv Barak Cenana) using 2,4 D plant growth regulator
Original languageOther
Title of host publicationKUMPULAN MAKALAH (PROCEEDING) SEMINAR NASIONAL BIODIVERSITAS VI
Subtitle of host publicationBiodiversitas untuk Pembangunan Berkelanjutan - Keanekaragaman Hayati Indonesia dan Perannya dalam Menunjang Kemandirian Bangsa
EditorsAlfiah Hayati, Dwi Winarni, H. Hery Purnobasuki, Ni Matuzahroh, Thin Soedarti, Eko Prasetyo Kuncoro
PublisherUniversitas Airlangga
ChapterII
Pages67 - 73
Number of pages1233
ISBN (Electronic)978-979-98109-5-3
Publication statusPublished - 3 Sep 2016
Externally publishedYes

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